Nucleotide sequences of the neuraminidase genes of influenza A/Leningrad/134/57 (H2N2) virus and two of its live,attenuated, cold-adapted variants |
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| Authors: | Alexander I Klimov Julia R Romanova Andrei Y Egorov Irina V Lukashok Irina V Kiseleva Galina I Alexandrova Nancy J Cox |
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| Institution: | (1) Influenza Branch, G-16, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd., 30333 Atlanta, GA;(2) Research Institute for Viral Preparations, Russian Academy of Medical Sciences, Moscow, Russia;(3) Research Institute for Experimental Medicine, Russian Academy of Medical Sciences, St. Petersburg, Russia |
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| Abstract: | The nucleotide sequences of the neuraminidase (NA) genes of the A/Leningrad/134/57 (H2N2) wild-type (Len/wt) virus as well as two of its live attenuated, cold-adapted (ca) variants, A/Leningrad/134/17/57 (Len/17) and A/Leningrad/134/47/57 (Len/47), were determined. In comparison with Len/wt, one nucleotide change (C-225 to A) was found in the NA gene of Len/17. This change codes for a Thr-to-Asn substitution at position 69 of NA. The NA gene of the more attenuated Len/47 ca virus has one silent (T-814 to C) and two coding nucleotide substitutions, C-78 to T (Ala-20 to Val) and C-225 to A (Thr-69 to Asn). These sequence data were used to design a PCR-restriction technique to determine the origin of the NA gene in candidate live, attenuated vaccine reassortants made by reassorting these ca strains with current field viruses.The nucleotide sequence data reported in this paper have been submitted to the GenBank nucleotide sequence database and have been assigned accession numbers L37329-L37331. |
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| Keywords: | influenza A virus NA gene nucleotide sequence PCR restriction analysis |
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