Suppression of elevated plasma interleukin-8 levels due to total ischemia and reperfusion of the small intestine by luminal perfusion with fetal bovine serum |
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Authors: | Yun Chen Wen-Tsung Hung Shih-Ming Chen Sheng-Hong Tseng |
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Institution: | (1) Department of Surgery, National Taiwan University Hospital, 7 Chung-Shan S. Rd., Taipei 100, Taiwan, Republic of China, CN |
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Abstract: | A previous study demonstrated that continuous enteric luminal perfusion of fetal bovine serum (FBS) protects the small intestine
from total ischemia/reperfusion injury (IRI) and increases the intestinal mass. In this study, we further investigated the
changes in plasma interleukin-8 (IL-8) level caused by total ischemia/reperfusion of the small intestine and the effect of
FBS on plasma IL-8 levels. A 3-h total ischemia was induced in a 15-cm segment of terminal ileum and then reperfusion was
instituted. Luminal perfusion of FBS was conducted via an osmotic minipump connected to the stomach through a fine polyethylene
tube, starting 3 days prior to total ischemia. The rats were killed after 10 and 30 min and 1 and 3 h of total ischemia, and
1, 6, and 12 h or 1, 2, and 3 days after initiation of reperfusion. Plasma IL-8 was measured by enzyme-linked immunosorbent
assay. The results were compared among the FBS-treated and untreated groups. The plasma IL-8 level was elevated from 1 h of
total ischemia to 6 h after initiation of reperfusion (P < 0.05) with a peak of 641.5 ± 36.9 pg/ml in the untreated group and 471.6 ± 42.2 pg/ml in the treated group. Luminal perfusion
of FBS significantly suppressed plasma IL-8 levels after 1 h of total ischemia and 1 h after initiation of reperfusion (P < 0.05). The results suggest that FBS might play a role in the treatment of total IRI of the small intestine.
Accepted: 21 March 2001 |
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Keywords: | Fetal bovine serum Interleukin-8 Total ischemia Reperfusion |
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