鼻咽癌患者血浆EBVDNA水平和VCA—IgA检测的临床意义

目的：探讨鼻咽癌患者血浆EBVDNA水平和VCA—IgA联合检测对鼻咽癌早期诊断的临床价值。方法：用荧光定量PCR方法检测血浆EBVDNA水平，常规免疫酶法检测VCA—IgA抗体滴度。结果：68例鼻咽癌患者中，EBVDNA阳性率95．59％，中位拷贝数93× 10^4 copies／ml，VCA—IgA抗体阳性率92．65％，抗体滴度≥1：20；其他头颈肿瘤36例中，EBVDVA阳性率5．56％，中位拷贝数为0copies／ml，VCA—IgA抗体阳率36．11％，阳性滴度均≤1：20；健康对照组EBVDNA阳性率为35．56％，其滴度除3例≥1：40外，余均≤1：20。鼻咽癌患者中EBVDNA和VCA—IgA抗体阳性率显著高于对照组。结论：进-步证实EBV与鼻咽癌有密切关系；EBVDNA水平和VCA—IgA抗体滴度联合检测，有助于鼻咽癌的早期辅助诊断。

Clinical significance of plasma EBV DNA and VCA -IgA for nasopharyngeal carcinoma

Objective ：To explore the clinical value of plasma EBV DNA and VCA -IgA lerel for earlier diagnose of nasopharyngeal carcinoma.. Methods： Using the method of PCR to test plasma EBV DNA and the method of immu- noenzyme to test VCA - IgA. Results： Among the 68 patients with nasopharyngeal carcinoma, positive percentage of EBV DNA was 95.59%, the number of median copies was 93 × 10^4 eopies/ml ; the positive percentage of VCA - IgA was 92.65%, the titer ≥1：20. Amony 36 patients with other head and neck tamors positive percentage of EBV DNA was 5.56% , the number of median copies was 0 copies/ml; the positive percentage of VCA - IgA was 36.11%, postive titer ≤ 1：20, the healthy comparison group＇s postive percentage was 35.56%, the gutta ≤ 1：20 with the ex- ception of 3 patients ≥ 1 ： 40. The positive percentage of the patient with nasopharyngeal carcinoma plasma EBV DNA and VCA -IgA was higher than that of comparison group signifcantly. Conclusion： The relationship between EBV and nasopharyngeal carcinoma is proved furthermore. Testing plasma EBV DNA and VCA - IgA is helpful for the earlier diagnose of the nasopharyngeal carcinoma.