硫酸镁对培养乳鼠心肌细胞缺氧/复氧损伤的保护作用

目的 研究硫酸镁对纯化培养乳鼠心肌细胞缺氧／复氧损伤的保护作用及其作用机制。方法 采用培养乳鼠心肌细胞建立缺氧／复氧损伤模型，实验分为五组：正常对照组、缺氧／复氧损伤模型组、缺氧／复氧损伤＋MgSO4（2．2、4．0、7．2mmol／L）组。分别用黄嘌呤氧化酶法测定细胞内SOD活性，硫代巴比妥酸法测定细胞内MDA含量，MTT染色法测定心肌细胞活力并测定培养液中LDH含量的变化。结果 硫酸镁能显著提高细胞内SOD的活性，降低MDA、LDH的含量并可提高心肌细胞的活力。结论 硫酸镁具有明显的抗缺氧／复氧损伤、保护心肌细胞的作用。

Protective Effects of Magnesium Sulfate on Hypoxia/reoxygenation Injury in Cultured Neonatal Rat Cardiomyoytes

Objective The aim of this study was to investigate the influences of magnesium sulfate on hypoxia/reoxygenation injury in cultured neonatal rat myocardial cell. Methods The model of hypoxia/reoxygenation injury was developed in primary-cultured neonatal rat cardiac myocytes.The cultured cells were divided into five groups,namely normal,H/R,H/R+MgSO_4(2.2,4.0,7.2mmol/L).The activities of corpuscle superoxide dismutase and the contents of serum malonicadehyde were measured,The incubation activity of cardiomyocytes were detected by MTT assay.The release of LDH in culture medium were also evaluated. Results Magnesium can increase the activity of SOD(P<0.01) and reduce the content of MDA and LDH,and can enhance the viability of myocytes.(P<0.01or P<0.05). Conclusions Magnesium reduces reoxygenation-induced injury in cardiomyocytes and is potentially mediated by attenuation of ROS generation,lipid peroxidation and elevation of the activity of SOD.