Whole-comparative genomic hybridization (W-CGH): 1. The quick overview of repetitive DNA sequences on a genome |
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Authors: | Miguel Pita José Luis Fernández Jaime Gosálvez |
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Affiliation: | (1) Unidad de Genética, Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, 28049 Madrid, Spain |
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Abstract: | We present a technique (W-CGH) based on Comparative Genomic Hybridization (CGH), but using whole DNA probes, which permits the identification of chromosomal polymorphisms related to highly repetitive DNA sequences that exist between the two genomes compared. The procedure employs two differently colored whole DNA probes from two different individuals that are mixed and hybridized to metaphase chromosomes. The method provides a simple way to map whole genome differences for highly repetitive DNA sequences between two individuals, since it does not require chromosome-specific probes. This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | comparative genomics heterochromatin human chromosomes in-situ hybridization fluorescence |
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