Alterations in corneal nerves in different subtypes of dry eye disease: An in vivo confocal microscopy study |
| |
| Institution: | 1. Center for Translational Ocular Immunology and Cornea Service, Department of Ophthalmology, Tufts Medical Center, Tufts University School of Medicine, Boston, MA, USA;2. Ocular Surface Imaging Center, Cornea & Refractive Surgery Service, Massachusetts Eye & Ear Infirmary, Department of Ophthalmology, Harvard Medical School, Boston, MA, USA;1. Center for Translational Ocular Immunology, USA;2. Cornea Service, Department of Ophthalmology, Tufts Medical Center, Tufts University School of Medicine Boston, MA, USA;3. Department of Ophthalmology, New England Eye Center, Tufts Medical Center, Tufts University School of Medicine, Boston, MA, USA;1. Department of Environmental Health Sciences, Yale School of Public Health, Yale University, 60 College Street, New Haven, CT, USA;2. Department of Cellular & Molecular Physiology, Yale School of Medicine, Yale University, New Haven, CT, USA;3. Bioinformatics Support Program, Cushing/Whitney Medical Library, Yale School of Medicine, New Haven, CT, USA;4. Department of Medicine, Yale University School of Medicine, New Haven, CT, USA;5. Veterans Affairs Connecticut Healthcare System, West Haven, CT, USA;6. Department of Ophthalmology & Visual Science, Yale School of Medicine, New Haven, CT, USA;7. Department of Pathology, Anschutz School of Medicine, University of Colorado, Aurora, CO, USA;8. Department of Biochemistry and Mass Spectrometry Research Center, Vanderbilt University School of Medicine, Nashville, TN, USA;9. Department of Clinical Pharmacy, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of Colorado Denver, Aurora, CO, USA;4. Graduate Institute of Bioelectronics and Biomedical Informatics, College of Electronic Engineering and Computer Science, National Taiwan University, Taipei, Taiwan;5. Department of Dentistry, Chung Shan Medical University and Chung Shan Medical University Hospital, Taichung, Taiwan |
| |
| Abstract: | PurposeTo evaluate corneal subbasal nerve alterations in evaporative and aqueous-deficient dry eye disease (DED) as compared to controls.MethodsIn this retrospective, cross-sectional, controlled study, eyes with a tear break-up time of less than 10 s were classified as DED. Those with an anesthetized Schirmer's strip of less than 5 mm were classified as aqueous-deficient DED. Three representative in vivo confocal microscopy images were graded for each subject for total, main, and branch nerve density and numbers.ResultsCompared to 42 healthy subjects (42 eyes), the 70 patients with DED (139 eyes) showed lower total (18,579.0 ± 687.7 μm/mm2 vs. 21,014.7 ± 706.5, p = 0.026) and main (7,718.9 ± 273.9 vs. 9,561.4 ± 369.8, p < 0.001) nerve density, as well as lower total (15.5 ± 0.7/frame vs. 20.5 ± 1.3, p = 0.001), main (3.0 ± 0.1 vs. 3.8 ± 0.2, p = 0.001) and branch (12.5 ± 0.7 vs. 16.5 ± 1.2, p = 0.004) nerve numbers. Compared to the evaporative DED group, the aqueous-deficient DED group showed reduced total nerve density (19,969.9 ± 830.7 vs. 15,942.2 ± 1,135.7, p = 0.006), branch nerve density (11,964.9 ± 749.8 vs. 8,765.9 ± 798.5, p = 0.006), total nerves number (16.9 ± 0.8/frame vs. 13.0 ± 1.2, p = 0.002), and branch nerve number (13.8 ± 0.8 vs. 10.2 ± 1.1, p = 0.002).ConclusionsPatients with DED demonstrate compromised corneal subbasal nerves, which is more pronounced in aqueous-deficient DED. This suggests a role for neurosensory abnormalities in the pathophysiology of DED. |
| |
| Keywords: | Corneal nerves Dry eye disease In vivo confocal microscopy DED"} {"#name":"keyword" "$":{"id":"kwrd0030"} "$$":[{"#name":"text" "_":"Dry eye disease IVCM"} {"#name":"keyword" "$":{"id":"kwrd0040"} "$$":[{"#name":"text" "$$":[{"#name":"italic" "_":"in vivo"} {"#name":"__text__" "_":" confocal microscopy TBUT"} {"#name":"keyword" "$":{"id":"kwrd0050"} "$$":[{"#name":"text" "_":"tear break up time |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
