| COPD大鼠膈肌组织泛素-蛋白酶体途径的研究 |
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| 引用本文： | 韩锋锋,徐卫国,罗勇,杨天芸,陈迎春,张悦.COPD大鼠膈肌组织泛素-蛋白酶体途径的研究[J].中国呼吸与危重监护杂志,2010,9(2):141-144. |
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| 作者姓名： | 韩锋锋 徐卫国 罗勇 杨天芸 陈迎春 张悦 |
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| 作者单位： | 上海交通大学医学院附属新华医院呼吸科,上海,200092 |
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| 基金项目： | 上海市自然科学基金资助项目 |
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| 摘 要： | 目的研究COPD大鼠膈肌组织内蛋白降解途径之一的泛素-蛋白酶体系统组成成分的变化及意义。方法采用单纯熏香烟法复制COPD大鼠动物模型。蛋白免疫印记（Westernblot）方法测定大鼠膈肌内E2-14k、蛋白酶体C8亚基的蛋白表达;逆转录-聚合酶链式反应（RT-PCR）技术检测膈肌内泛素和蛋白酶体C2亚基的mRNA表达。结果Westernblot分析结果显示,COPD大鼠膈肌组织中E2-14k的蛋白表达较正常对照组显著增强（0.81±0.28比0.50±0.25,P〈0.05）,蛋白酶体C8亚基的蛋白表达变化不明显（P〉0.05）;RT-PCR分析结果显示,膈肌组织中泛素的mRNA表达（IOD值）较对照组显著增加（0.89±0.20比0.50±0.15,P〈0.05）,蛋白酶体C2亚基的mRNA表达与正常对照组比较无明显差异（P〉0.05）。结论COPD大鼠膈肌组织内泛素-蛋白酶体降解途径被激活,可能是引起蛋白降解的重要因素之一。
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| 关 键 词： | 慢性阻塞性肺疾病 膈肌 泛素-蛋白酶体途径 |
The Ubiquitin-Proteasome Pathway Is Activated in the Diaphragm of COPD Rats |
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| HAN Feng-feng,XU Wei-guo,LUO Yong,YANG Tian-yun,CHEN Ying-chan,ZHANG Yue.The Ubiquitin-Proteasome Pathway Is Activated in the Diaphragm of COPD Rats[J].Chinese Journal of Respiratory and Critical Care Medicine,2010,9(2):141-144. |
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| Authors: | HAN Feng-feng XU Wei-guo LUO Yong YANG Tian-yun CHEN Ying-chan ZHANG Yue |
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| Institution: | HAN Feng-feng,XU Wei-guo,LUO Yong,YANG Tian-yun,CHEN Ying-chun,ZHANG Yue.Departmant of Respiratory Medicine,Xinhua Hospital,School of Medicine,Shanghai Jiaotong University.Shanghai,200092,China |
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| Abstract: | Objective To study the role of ubiquitin-proteasome pathway in diaphragm of COPD rats.Mathods Thirty rats were divided into a normal control group and a COPD group.COPD model was established by exposure to cigarette smoke for three months.The protein levels of E2-14k and proteasome subunit C8 in diaphragms were measured by Western blot.The mRNA levels of ubiquitin and proteasome subunit C2 in diaphragms were measured by means of realtime polymerase chain reaction(RT-PCR).Results Compared with the control gr... |
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| Keywords: | Chronic obstructive pulmonary disease Diaphragm Ubiquitin-proteasome pathway |
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