共培养诱导人脐带间充质干细胞(hUCMSCs)向牙周膜细胞(hPDLs)分化的体外实验研究

目的:研究人脐带间充质干细胞(hUCMSCs)向人牙周膜细胞(hPDLs)分化的可能性。方法:原代培养获得hUCMSCs和hPDLs;应用Transwell小室将两种细胞进行非接触式共培养;使用流式细胞仪检测共培养过程中hUCMSCs干细胞标记物CD146、SOX2、SSEA和Stro-1的变化情况;通过免疫荧光技术和Western Blot技术检测共培养过程中波形蛋白(Vimentin)在hUCMSCs中的变化情况。结果:共培养过程中hUCMSCs中的CD146、SOX2、SSEA和Stro-1持续降低;而Vimentin持续增高。结论:通过与hPDLs共培养,可以成功地将hUCMSCs诱导分化成为牙周膜样细胞,进一步证明了hUCMSCs可用于牙周损伤修复的可能。

Differentiation of Human Umbilical Cord Mesenchymal Stem Cells into Human Periodontal Ligament Cell by Co-culture in Vitro

Objective: To investigate the ability of human umbilical cord mesenchymal stem cells (hUCMSCs) differentiation into human periodontal ligament cells (hPDLs) by co-culture in vitro. Methods: The hUCMSCs and hPDLs were obtained by primary culture. hUCMSCs and hPDLs were indirectly cocultured in transwell plates. The stemness markers such as CD146, sex determining region Y-Box 2 (SOX2), stage-specific embryonic antigen (SSEA), and Stro-1 changes were detected by flow cytometry after coculture for 0, 1 and 3 weeks, respectively. The Vimentin expression was determined by immunofluorescence stain and Western blot. Results: During the co-culture process, the expression of stemness markers in hUCMSCs decreased gradually, while the Vimentin expression was on the contrary. Conclusion: The hUCMSCs had the potential to differentiate into hPDLs under co-culture condition, which suggested that it could be used for periodontal repair in the future.