RNA-Seq在老年神经退行性疾病研究中的应用

阿尔茨海默病及帕金森病是老年人最常见的两种神经退行性疾病，但其发病机制及治疗是研究的热点。随着高通量测序技术的进步及成本的下降，RNA-Seq也成为神经退行性疾病机制研究及生物标志物发现的有力手段。RNA-Seq相对于microarray具有高灵敏度、高准确性、高重复性以及噪声低等优势，在阿尔茨海默病及帕金森病研究中有较为广泛的应用，包括检测差异表达基因，可变剪接、新长链非编码RNA预测分析和miRNAs调控等，但是容易受病理复杂性及样本等因素影响。目前阿尔茨海默病及帕金森病转录组研究相比于癌症等还不够深入，在临床诊断及治疗应用还面临较大挑战。但是随着新技术及新方法的发展，RNA-Seq将进一步推动神经退行性相关疾病的研究和临床转化。

Application of RNA-Seq in the study of neurodegenerative diseases

Alzheimer's disease(AD) and Parkinson's disease(PD) are the two most common neurodegenerative diseases of the aged, and the pathogenesis and treatment are the hot spots in the current study.With the advance of high-throughput sequencing technology and the decline of cost, RNA sequencing (RNA-Seq) has also become a powerful method for the neurodegenerative disease mechanism research and biomarker discovery. Compared with microarray,RNA-Seq has the advantages of high sensitivity, high accuracy, high repeatability and low noise, and has a wider application in the study of AD and PD, including detection of differentially expressed genes, alternative splicing, new lncRNA predictive analysis and miRNAs regulation. Influenced by the pathological complexity and specimens,the regulatory mechanism research of AD and PD is not deep enough,and we also face great challenge in clinical diagnosis and treatment application. But with the development of new technologies and methods, RNA-Seq will further promote the research and clinical transformation of neurodegenerative diseases.