凝溶胶蛋白gelsolin对人牙髓细胞增殖及迁移的影响

目的研究凝溶胶蛋白gelsolin（GSN）对人牙髓细胞（hDPC）增殖及迁移的影响。 方法激光共聚焦检测gelsolin在hDPC中的表达。以小干扰RNA（siRNA）沉默hDPC中的gelsolin，CCK-8法检测细胞增殖；流式细胞仪检测细胞凋亡及细胞周期；Transwell实验观察细胞的迁移情况。采用单因素方差分析数据。 结果激光共聚焦显示gelsolin普遍表达于hDPC胞浆。沉默gelsolin，hDPC的增殖率在24、48、72 h时分别下降47.8%（F= 6.182，P= 0.035）、48.9%（F= 5.520，P= 0.044）、64.1%（F= 15.440，P= 0.004）；流式结果显示干扰gelsolin，hDPC的凋亡率增加约4.5%（F= 21.162，P= 0.002），而细胞周期未发生明显阻滞，S期细胞比例升高约5%（F= 4.526，P>0.05），G1期细胞比例下降约8%（F= 4.743，P>0.05）；Transwell结果显示，24 h内hDPC的迁移能力降低约60%（F= 12.781，P<0.001）。 结论gelsolin对hDPC的增殖及迁移具有调控作用。

The effects of gelsolin on the proliferation and migration of human dental pulp cells

ObjectiveTo investigate the effects of gelsolin on the proliferation and migration of human dental pulp cells (hDPCs) . MethodsThe expression of gelsolin in hDPCs was examined by laser confocal microscope. Small interfering RNA were transfected into hDPCs to knock down gelsolin mRNA. The proliferation and migratory abilities of hDPCs after gelsolin siRNA transfection were measured respectively by CCK-8 assay and Transwell assay. Cell cycle and apoptosis analysis were conducted by flow cytometry. Data were analyzed using One-Way ANOVA procedure. ResultsConfocal images indicated that gelsolin was widely expressed in the cytoplasm of hDPCs. Silencing of GSN in hDPCs significantly hindered cell proliferation by approximately 47.8% (F = 6.182, P = 0.035) , 48.9% (F= 5.520, P= 0.044) , 64.1% (F= 15.440, P= 0.004) followed by 24, 48, and 72 h culture in growth medium, which might be resulted from cell apoptosis induction instead of cell cycle arrest. The number of cells transferred through the Transwell membrane in si-GSN hDPCs was dramatically reduced by almost 60% (F= 12.781, P<0.001) when compared with controls. ConclusionGelsolin plays a regulatory role in the proliferation and migration of human dental pulp cells.