新疆木垒县哈萨克族慢性牙周炎患者血清与唾液中IL-6、IL-17的表达及相关性分析

目的:研究哈萨克族慢性牙周炎(chronic periodontitis,CP)患者血清和唾液中白细胞介素17(interleukin 17, IL17 )、白细胞介素6(interleukin 6, IL6 ) 的活性表达及相关性分析。方法:选择哈萨克族慢性牙周炎患者192例,健康人100例,记录临床牙周检查指标,采集非刺激性全唾液及外周血液各5 mL。采用抗体夹心ELISA法测定CP患者与健康人群唾液及血液中IL-17、IL-6总量和浓度。结果:牙周炎患者的唾液IL-6、IL-17浓度要显著高于血清IL-6、IL-17。在唾液中IL-6与IL-17彼此呈中度正相关性,且二者与牙周临床指标均为高度正相关关系。结论:唾液IL-6、IL-17在哈萨克族慢性牙周炎患者的表达水平明显增高,且与病情严重程度相关,可作为萨克族慢性牙周炎患者的诊断标志物,对二者的相关性可从基因学方面进行下一步研究。

Correlation between Chronic Periodontitis and Levels of IL-6 as well as IL-17 in Serum and Saliva from Kazak in Mulei,Xinjiang, China

Objective: To investigate interleukin 17 (IL-17) and interleukin 6 (IL-6) levels in serum and saliva of patients with chronic periodontitis from Kazak, and their correlation with the clinical periodontal index. Methods: A total of 292 subjects from Kazak were included in this study (192 patients with chronic periodontitis and 100 clinically healthy subjects). Five milliliter non-stimulated saliva and serum samples were collected. Enzyme linked immunosorbent assay (ELISA) kit was employed to determine the levels of IL-17 and IL-6. Results: Concentrations of IL-6 and IL-17 in saliva were significantly higher than those in serum from the same patient with chronic periodontitis. There was a moderately positive correlation between concentrations of IL-6 and IL-17 in saliva. Additionally, both their levels showed a positive correlation with the severity of chronic periodontitis. Conclusion: The saliva levels of IL-6 and IL-17 showed significantly increased expression in Kazak patients with chronic periodontitis and were associated with the severity of disease. This suggests that saliva IL-6 and IL-17 levels may be used as indicators of chronic periodontitis in patients from Kazak.