人白细胞介素18对肺癌A549细胞生物学功能的影响

目的 探讨人白细胞介素18（interleukin 18，IL-18）对肺癌A549细胞生物学功能的影响。方法 将IL-18重组质粒及其空载对照质粒转染肺癌A549细胞，用抗生素加压筛选阳性细胞，构建稳定表达IL-18质粒及空载质粒的肺癌细胞，将构建好的稳定过表达IL-18细胞和空载对照细胞分别命名为IL18-A549细胞和NC-A549细胞。采用细胞计数法、流式细胞术、划痕实验和transwell迁移、侵袭实验检测IL-18过表达对肺癌A549细胞增殖、凋亡、运动、迁移和侵袭能力的影响。结果 成功构建稳定过表达IL-18肺癌细胞IL18-A549。与空载对照细胞NC-A549相比，过表达IL-18可促进肺癌A549细胞增殖（P＜0.05），但对细胞早期、晚期及总凋亡率无明显影响（P＞0.05）。IL18-A549细胞的划痕愈合率为（62.0±5.7）%，高于NC-A549细胞的（31.2±3.7）%，两者比较差异有统计学意义（P＜0.05）；IL18-A549细胞和NC-A549细胞发生迁移的细胞数目平均为48个和9个，发生侵袭的细胞数目平均为33个和11个，两者差异均有统计学意义（P均＜0.05）。结论 过表达IL-18可促进肺癌A549细胞增殖、运动、迁移和侵袭，IL-18可能是肺癌转移过程中的关键因子。

Influence of IL-18 on biological function of human lung cancer cell A549

Objective To investigate the biological functions of IL-18 on the human lung cancer cell A549，including its role in cancer-related processes. Methods A plasmid expressing IL-18 or a control plasmid were transfected into the A549 human lung cancer cell line，and antibiotic selection was used to identify stable transfectants. The resulting respective cell lines，IL18-A549 and NC-A549，were compared in terms of proliferation， apoptosis， movement，invasion and migration. Results The IL18-A549 cell line stably expressed IL-18， and comparison with the NC-A549 cell line suggested that IL-18 promotes proliferation（P<0.05）. The rate of healing in a wound healing assay was significantly higher for IL18-A549 cells（62.0±5.7）% than for control cells （31.2±3.7）%（P<0.05），and the percentages of cells showing migration and invasion in a transwell assay were significantly higher for IL18-A549（48% and 33%） than for controls （9% and 11%；P<0.05）. Flow cytometry showed similar rates of early，late and total apoptosis between IL-18 transfectants and control cells. Conclusion IL-18 promotes proliferation，movement，invasion and migra-tion of A549 cells，and may play a key role in human lung cancer. Inhibiting IL-18 function may be useful for treating lung cancer.