玉郎伞水提物对心肌缺血再灌注损伤心肌组织ATP酶和凋亡蛋白的影响

目的:研究玉郎伞水提物(WYLS)对大鼠离体心脏缺血再灌注(I/R)损伤心肌组织Na⁺-K⁺-ATP酶、Ca²⁺-ATP酶和凋亡蛋白的影响。方法:采用Langendorff离体心脏灌流法,停灌30 min再灌30 min建立大鼠心肌缺血再灌注损伤模型。观察WYLS对心肌组织Na⁺-K⁺-ATP酶、Ca²⁺-ATP酶活性的影响、心肌组织病理学变化、凋亡相关蛋白Bcl-2和Bax表达的影响。结果:与I/R组比较,WYLS高剂量组Na⁺-K⁺-ATP酶、Ca²⁺-ATP酶的活性明显增加(P<0.05或P<0.01),心肌受损程度明显减轻(P<0.05)。同时,WYLS高剂量组心肌Bax蛋白表达明显降低(P<0.01),Bcl-2蛋白表达无明显影响(P>0.05),但Bcl-2/Bax的比率明显增加(P<0.05)。结论:WYLS能减轻大鼠心肌I/R损伤,作用机制可能与减轻钙超载、抑制某些凋亡相关蛋白表达有关。

Effects of aqueous extracts of Yulangsan on ATPase and apoptosis-related protein in myocardial tissues with myocardial ischemia reperfusion injury

OBJECTIVE To study the effects of aqueous extracts of Yulangsan (WYLS) on the activities of Na⁺-K⁺-ATPase and Ca²⁺-ATPase and the apoptosis-related proteins in myocardial tissues with myocardial ischemia-reperfusion (I/R) injury in isolated rat hearts.METHODS Langendorff-perfusion isolated rat hearts were prepared. Myocardial ischemia reperfusion injury was induced by 30 minutes of global ischemia followed by 30 minutes of reperfusion. The activities of Na⁺-K⁺-ATPase and Ca²⁺-ATPase in myocardial tissues were determined. The pathological changes of myocardium and the expressions of apoptosis-related proteins Bcl-2 and Bax were analyzed.RESULTS Compared with I/R group, the activities of Na⁺-K⁺-ATPase and Ca²⁺-ATPase significantly increased in high dose WYLS group (P<0.05 or P<0.01). The severity of myocardial injury in high dose WYLS group evidently lessened (P<0.01). The expression of Bax significantly decreased (P<0.01) and the ratio of Bcl-2/Bax significantly increased in high dose WYLS group (P<0.05).CONCLUSION WYLS can protect against myocardial ischemia reperfusion injury, the mechanism of which is its effects on reducing calcium overload and down regulating the expression of apoptosis-related protein.