RhoGDI2蛋白的高表达与结直肠癌侵袭和转移的相关性分析

目的通过检测RhoGTP酶解离抑制因子2（RhoGTPase dissociation inhibitor 2，RhoGDI2）在结直肠癌和癌旁组织中的表达并结合临床病理特点进行分析，探讨RhoGDI2的蛋白表达水平与结直肠癌侵袭转移的关系及在肿瘤发生发展中的作用。 方法选取山东省医学科学院附属医院2012年07月至2014年07月采取手术切除的153例结直肠癌病例，采用免疫组织化学检测153例肿瘤组织标本和癌旁标本中RhoGDI2蛋白的表达水平。 结果RhoGDI2蛋白在结肠癌组织中呈阳性表达，且主要在胞浆中表达；但在癌旁正常组织中无阳性表达（57.5% vs 0.00%）。结直肠癌组织中RhoGDI2蛋白的表达在低分化组显著高于高中分化组（χ²=2.090，P<0.05）。RhoGDI2的阳性表达与结直肠肿瘤的大小呈正相关，肿瘤越大阳性表达率越高（χ²=4.293，P<0.05），并随着肿瘤浸润深度阳性表达率增加（χ²=1.711，P<0.05），其在有淋巴结转移者表达显著高于无淋巴结转移者（χ²=5.925，P<0.05）、有远处转移者显著高于无远处转移者（χ²=5.519，P<0.05），并且与临床分期密切相关，分期较晚的显著高于分期较早的（χ²=5.683，P<0.05）。RhoGDI2在结直肠癌中的阳性表达与年龄（<60 years vs ≥60 years）（χ²=0.054，P>0.05），性别（χ²=0.037，P>0.05）无相关性。 结论RhoGDI2在结直肠癌中高表达，并且与结直肠癌的局部侵袭和远处转移呈正相关。RhoGDI2可能在结直肠的发生发展和侵袭转移过程中起着重要的作用。

Overexpression of RhoGDI2 protein correlates with tumor invasion and metastasis in colorectal cancer

ObjectiveTo detect the expression of Rho GDP dissociation inhibitor 2 (RhoGDI2) and analyze the function of RhoGDI2 in the colorectal cancer. To discuss the role of RhoGDI2 in the developmetal process of colorectal cancer. MethodsImmunohistochemistry was used to detect RhoGDI2 protein in 153 cases of colorectal cancer and adjacent tissue specimens. ResultsSpecimens from 153 cases of colorectal cancer were selected from Affiliated Hospital of Shandong Academy of Medical Sciences between July 2012 and July 2014. RhoGDI2 was expressed mainly in colorectal cancer, mainly in the cytoplasm. The positive rate of RhoGDI2 protien expression was significantly higher in cancer tissue than in normal colorectal tissue (57.5 % vs 0.00%). RhoGDI2 expression in colorectal cancer tissue with the low differentiation group was significantly higher than in the high and moderate differentiation group. The expression of RhoGDI2 protein in colorectal cancer was significantly correlated with tumor size (χ²=4.293, P<0.05), grade of differentiation (χ²=2.090, P<0.05), depth of invasion (χ²=1.711, P<0.05), lymph node metastasis (χ²=5.925, P<0.05), distant metastasis (χ²=5.519, P<0.05), and clinical stage (χ²=5.683, P<0.05). RhoGDI2 expression in colorectal cancer was no correlated with gender (χ²=0.037, P>0.05)、age (<60 years vs≥60 years) (χ²=0.054, P>0.05). ConclusionsRhoGDI2 expression was positively correlated with the invasion and metastasis of colorectal cancer, and RhoGDI2 may play an important role in the occurrence and development of colorectal cancer.