葡萄籽原花青素对复发性结肠炎大鼠结肠组织中细胞因子的影响

目的:观察葡萄籽原花青素(GSPE)对复发性结肠炎大鼠结肠组织中细胞因子的影响,探讨其治疗复发性结肠炎的作用机制。方法:大鼠随机分为正常对照组、复发性结肠炎模型组、阳性对照(柳氮磺吡啶,SASP)组和GSPE低、中、高剂量组(100,200,400 mg·kg^-1)。大鼠直肠给予80 mg·kg^-1 2,4,6-三硝基苯磺酸(TNBS)-50%乙醇溶液建立溃疡性结肠炎模型,在第16天时,用30 mg·kg^-1 TNBS-50%乙醇溶液诱导复发性结肠炎模型。大鼠第2次致炎24 h后,分别应用SASP及不同剂量的GSPE对其进行治疗。连续灌胃给药7 d后处死所有大鼠,酶联免疫法(ELISΑ)检测大鼠结肠组织中白细胞介素1β(IL-1β)、IL-4、IL-6 及IL-10的含量。结果:给予不同剂量的GSPE治疗后,大鼠结肠组织中IL-1β 和 IL-6含量与复发性结肠炎模型组比较,显著降低,而IL-4和IL-10含量均显著升高(P<0.01)。结论:GSPE 对复发性结肠炎的治疗作用可能是通过上调大鼠结肠组织中抗炎细胞因子IL-4和IL-10水平,同时抑制结肠组织中促炎细胞因子IL-1β和IL-6水平实现的。

Effects of proanthocyanidins from grape seeds on levels of cytokines in colon tissues of rats with recurrent colitis

OBJECTIVE To investigate expression levels of cytokines of proanthocyanidins from grape seeds (GSPE) in rats with recurrent colitis induced by twice administered 2, 4, 6-trinitrobenzene sulfonic acid (TNBS) and probe into the underlying mechanism.METHODS Rats were randomly divided into normal control group, recurrent colitis group, positive control (Sulfasalazine, SASP) group, and GSPE groups, which were stratified into low dose (100 mg·kg^-1), medium dose (200 mg·kg^-1) and high dose (400 mg·kg^-1). Recurrent colitis was induced in rats by rectal administration of 80 mg·kg^-1 TNBS dissolved in 50% ethanol. In the 16th day, rats were again administered 30 mg·kg^-1 TNBS into colon to induce recurrent colitis. Rats were killed after SASP and different doses of GSPE for 7 days. Enzyme-linked immunosorbent assay was used to measure expression levels of interleukin (IL)-1β, IL-4, IL-6, and IL-10 in colon tissues. RESULTS After treated with GSPE, levels of IL-1β and IL-6 in colon tissues were significantly decreased compared with recurrent colitis group. Meanwhile, levels of IL-4 and IL-10 were significantly higher in GSPE groups than in recurrent colitis group.CONCLUSION GSPE can alleviate inflammatory reactions during recurrent colitis through increasing production of IL-4 and IL-10, and inhibiting production of IL-1β and IL-6 in colon tissues.