hBMP-7基因修饰犬骨髓基质干细胞复合珊瑚羟基磷灰石修复下颌骨缺损

目的:构建携带人骨形态发生蛋白7(hBMP-7)基因的重组腺病毒载体,体外转染犬骨髓基质干细胞(dMSCs)复合珊瑚羟基磷灰石(coral hydroxyapatite, CHA),观察其修复下颌骨缺损的效果。方法:利用AdEasy腺病毒表达系统,体外构建高效表达hBMP-7重组腺病毒载体,转染dMSCs 与珊瑚羟基磷灰石支架材料复合,再分别植入取材动物的自体下颌骨缺损区,分别于4周和8周取材观察成骨情况。结果:大体观察、X线检查及组织学观察均发现构建组织工程骨在祼鼠皮下及骨缺损处均有明显新骨组织形成。结论:构建携带人骨形态发生蛋白7(hBMP-7)基因的重组腺病毒载体,体外转染dMSCs后复合珊瑚羟基磷灰石有较多量骨组织形成,可有效修复下颌骨缺损。关键词] 人骨形态发生蛋白7 骨髓基质干细胞 腺病毒 组织工程

Construction of hBMP-7 Gene Recombinant Adenovirus and Its Expression after Infecting dMSCs.

Objective: To observe the repair of mandibular defects with tissue engineering bone using coral hydroxyapatite and dog marrow stromal cells (dMSCs) which was transfected by recombinant adenovirus vector in vitro carrying human bone morphogenetic protein-7 (hBMP-7) gene. Methods: After the compound of coral hydroxyapatite composite scaffold material and dMSCs transfected with high-level expression hBMP-7 adenovirus vector constructed by AdEasy adenovirus expression system was implanted in mandibular defects of dogs, the reparation situation of mandibular defection was observed 4 weeks and 8 weeks after operation respectively. Results: Significant new bone formation was found in the naked mouse skin and bone defect by gross observation, X-ray examination and histological observation of the constructed tissue engineering bone. Conclusion: Constructed tissue engineering bone carrying hBMP-7 gene was found to format more volume bone tissue, and could effectively repair mandibular defect.