雌激素受体阴性乳腺癌中神经胶质瘤致病因子1、基质金属蛋白酶-9的表达及其临床意义

 目的 探讨雌激素受体(estrogen receptor ,ER)阴性乳腺癌中的神经胶质瘤致病因子1(glioma-associated oncogene homolog 1,Gli1)及基质金属蛋白酶-9(matrix metalloproteinases-9,MMP-9)的表达及临床意义。方法 用免疫组化Envision法检测ER阴性乳腺癌组织中Gli1、MMP-9、 CD34的表达情况, 分析Gli1、MMP-9表达与CD34、肿瘤大小、淋巴转移、临床TNM分期、HER-2表达的关系。结果 ER阴性乳腺癌组织中,Gli1阴性(-)、低表达(+)、中表达(++)及高表达(+++)组的CD34分别为48.83±7.53、60.69±10.02、64.68±12.92和75.91±11.30; MMP-9分别为48.15±8.44、61.69±10.71、67.76±14.03、73.11±12.78,组间比较差异均有统计学意义(P<0.05)。ER阴性乳腺癌组织中Gli1、MMP-9表达与肿瘤大小、临床TNM分期呈正相关(P<0.05)。ER阴性乳腺癌组织中Gli1与MMP-9表达存在一定正相关性(r=0.462,P<0.01)。结论 Gli1与MMP-9表达呈正相关性,推测Gli1是ER阴性乳腺癌增殖途径中重要的调控节点之一。

Expression of Gli1, MMP-9 in estrogen-independent breast cancer and its clinical significance

Objective To study the expression and clinical significance of glioma-associated oncogene homolog 1 (Gli1) and matrix metalloproteinases-9 (MMP-9) in estrogen-independent breast cancer. Methods The expression of Gli1 protein was examined by Envision immunohistochemistry, Gli1, MMP-9 and CD34 expression were examined in ER-negative breast cancer. The relationships between them and CD34, tumor size, TNM stages, lymph node metastasis, HER-2 expression in ER-negative breast cancer were analyzed. Results The expression of CD34 was enhanced by the expression of Gli1 and MMP-9 in ER-negative group(CD34 expression were 48.83±7.53,60.69±10.02,64.68±12.92,75.91±11.30 in different group of Gli1;CD34 expressions were 48.15±8.44,61.69±10.71,67.76±14.03,73.11±12.78 in different group of MMP-9), with significant differences (P<0.05). The expression of Gli1 and MMP-9 were correlated with tumor size and clinical TNM stages in ER-negative breast cancer (P<0.05). The expression of MMP-9 was also correlated with lymphatic metastasis (P<0.05). The correlation existed between the expression of Gli1 and MMP-9 in ER-negative breast cancer(r=0.462, P<0.01). Conclusions Clinical positive correlation exists between the expression of Gli1 and MMP-9, Gli1 is an important regulating point in estrogen-independent breast cancer growth way.