桐花树根多酚提取物体外α-糖苷酶抑制活性与抗氧化能力

目的:研究桐花树根多酚提取物对α-糖苷酶的抑制作用与抗氧化活性。方法:采用PNPG法测定α-葡萄糖苷酶抑制活性,采用羟基自由基清除体系、DPPH·自由基清除体系、对脂质体过氧化活性测定评价桐花树根多酚的抗氧化活性。结果:桐花树根多酚提取物对α-葡萄糖苷酶有一定的抑制活性,IC₅₀为37.75μg·ml^-1。桐花树根多酚提取物表现出较强的抗氧化性能,清除DPPH的IC₅₀为0.618 mg·ml^-1,清除羟自由基的IC₅₀为0.0002 mg·ml^-1,对脂质体过氧抑制能力的IC₅₀约为0.585 mg·ml^-1。结论:桐花树根多酚提取物具有作为新型α-葡萄糖苷酶抑制剂与抗氧化剂的药用开发价值。

Study on in vitro inhibitory and antioxidant activities of polyphenol extracts from Aegiceras corniculatum roots on α-glucosidase

OBJECTIVE To study inhibitory and antioxidant activities of polyphenol extracts from Aegiceras corniculatum roots on α-glucosidase.METHODS PNPG was used to determine α-glucosidase inhibiting activity. Hydroxyl radical scavenging system, DPPH·radical scavenging system and lipid peroxidation activity assay were used to evaluate antioxidant activity.RESULTS Polyphenol extracts from Aegiceras corniculatum roots showed certain degrees of α-glucosidase inhibitory activities with IC₅₀ of 37.75μg·ml^-1, and also strong antioxidant activity. IC₅₀ values were 0.618, 0.0002 and 0.585 mg·ml^-1 for DPPH clearing, hydroxyl radicals scavenging and lipid peroxidation inhibiting, respectively.CONCLUSION Polyphenol extracts from Aegiceras corniculatum roots have medicinal development values as a new α-glucosidase inhibitor and antioxidant。