乳牙根尖周病损组织细菌组成与临床症状的关系

目的:检测乳牙根尖周病损组织中菌群构成,分析检出细菌种类与临床症状的关系。方法:收集31例乳牙根尖周炎患儿的根尖周病损组织,采用16S rDNA 克隆测序法检测样本中菌群构成,统计分析菌种检出率与有无自发疼、咀嚼疼及窦道的关系。结果:31个乳牙根尖周病损组织样本共获得2057个可分析序列,鉴定出268个菌种或基因型,从属于9个菌门。检出率最高的具核梭杆菌(74.19%)、隐蔽小杆菌(67.74%)和纤细弯曲杆菌(64.52%)与各临床症状间无明显关联,普氏坦纳菌在有自发痛样本中检出率显著增高,口普氏菌和副溶血嗜血杆菌在有咀嚼痛样本中的检出率显著增高,变黑普氏菌和短优杆菌存在的样本发生窦道的几率显著增高,两种未培养韦荣氏菌的检出率在有咀嚼痛的样本中显著减少。结论:乳牙慢性根尖周病损是包含多种细菌的混合感染,普氏菌、优杆菌及韦荣氏菌与临床症状有一定的关系。

Relationship between Clinical Characteristics and Bacterial Flora in Periapical Lesions of Deciduous Teeth.

Objective: To detect the bacterial components in periapical lesions of deciduous teeth and analyze the relationship between the bacterial flora and clinical symptoms. Methods: 31 periradicular lesion samples of deciduous teeth diagnosed as periapical periodontitis were collected. DNA was extracted and analyzed using 16S rDNA cloning and sequencing for identification of bacteria. Chi-square was chosen to compare the detection rate of bacteria with different symptoms, including spontaneous pain, chewing pain and sinus. Results: 268 phylotypes from 2057 clones were identified belonging to nine phyla. The three most prevalent bacteria, Fusobacterium nucleatum (74.19%) , Dialister invisus (67.74%) and Campylobacter gracilis (64.52%)showed no relationship with any symptoms. Prevotella tannerae was detected to significantly increase in samples with spontaneous pain, so did Prevotella oris and Haemophilus parahaemolyticus in the group of chewing pain, and Prevotella nigrescens, Eubacterium brachy in samples with sinus. Two uncultured Veillonella sp. were significantly fewer in the samples of chewing pain. Conclusion: Periapical lesions in deciduous teeth contained polymicrobial. Prevotella, Eubacterium and Veillonella had certain relations with clinical symptoms.