不同加载频率拉应力对髁突软骨细胞增殖分化的影响

目的:探讨不同加载频率的拉应力对髁突软骨细胞增殖、分化的影响。方法:体外分离2周龄新西兰兔髁突软骨细胞,对细胞施加2 h/d,为期3 d的不同频率(0 Hz、0.1 Hz、0.5 Hz、1 Hz)的拉应力。采用CCK8检测细胞增殖水平,实时荧光定量RT-PCR检测Sox9、Runx2、VEGF的表达。结果:细胞增殖水平在静止性(0 Hz)组显著高于周期性加力(0.1~1 Hz)组和对照组;Sox9、Runx2、VEGF表达在0.5 Hz组和1 Hz组显著高于静止性(0 Hz)组和对照组。结论:不同加载频率拉应力对髁突软骨细胞增殖分化有差异性影响。

Effect of Different Frequency Tensile Stress on the Proliferation and Differentiation of Mandibular Condylar Chondrocytes

Objective: To investigate the effect of different frequency of tensile stress on the proliferation and differentiation of mandibular condylar chondrocytes. Methods: Mandibular condylar chondrocytes (MCC) from two-week-old New Zealand rabbits were cultured in vitro under different frequency(0.1Hz,0.5Hz,1Hz) tensile stress for a period of 2 h per day for 3 days .The proliferation of articular chondrocyte was determined by CCK-8 assay. The gene expression (Sox9, Runx2, VEGF )was analyzed using real-time RT-PCR. Results: The static(0HZ) tensile stress had a significant stimulate on the chondrocyte proliferation compared with periodic tensile stress(0.1-1HZ)and the control group. The expression of Sox9, Runx2 and VEGF under 0.5HZ and 1HZ was significantly higher than the static(0HZ) tensile stress and the control group. Conclusion: There are different effects on the proliferation and differentiation of MCC under different frequency tensile stress.