CCND1、ORAOV1、ERCC1在舌鳞状细胞癌中的表达和临床意义

目的:通过观察 CCND1、ORAOV1、ERCC1在舌鳞状细胞癌中的表达情况,探讨CCND1、ORAOV1、ERCC1与舌癌发生、发展的相关性。方法:应用免疫组织化学染色方法检测38例舌鳞状细胞癌组织芯片及4例正常舌组织芯片中CCND1,ORAOV1,ERCC1蛋白表达情况,并分析其与临床病理特征的关系。结果:舌癌组织中CCND1蛋白的阳性表达率明显高于正常舌组织(P<0.05),但其过表达水平与正常舌组织相比无统计学差异;CCND1阳性表达率及过表达水平与患者年龄、性别、肿瘤组织学分级及肿瘤有无淋巴结转移之间无明显相关。ORAOV1在舌癌组织中的表达明显升高(P<0.05),且与肿瘤有无淋巴结转移之间显著相关(P<0.05);ORAOV1在舌癌中的过表达水平与对照组相比无明显差异,但随着肿瘤分化程度的降低ORAOV1的过表达水平显著上升(P<0.05)。ERCC1蛋白在舌鳞状细胞癌组织中的阳性表达率及过表达水平均明显低于正常舌组织(P<0.05),并均与肿瘤有无淋巴结转移之间显著相关(P<0.05);且与高、中分化组相比,低分化组ERCC1蛋白过表达水平上升,但无统计学差异。结论:在舌鳞状细胞癌组织中CCND1和ORAOV1显著高表达,ERCC1表达显著降低,三者的检测对早期诊断舌癌具有指导意义。

Expression and Clinicopathological Significance of CCND1, ORAOV1 and ERCC1 in Tongue Squamous Cell Carcinoma.

Objective: To study the expression of CCND1, ORAOV1 and ERCC1 genes in tongue squamous cell carcinoma(TSCC), and investigate their associations with the clinicopathological features. Methods: Expression of CCND1, ORAOV1 and ERCC1 were analyzed in tissue microarrays of 38 TSCC patients and 4 cases of normal tongue tissue by immunohistochemistry. Results: The expression of CCND1 was significantly higher in TSCC(P<0.05), while the level of CCND1 overexpression in TSCC showed no significant difference compared with that in normal tongue tissues. There were no correlations between the intensities of CCND1 and some tumour parameters, such as age, gender, histological grade, and lymph node metastasis. The expression of ORAOV1 in TSCC was significantly higher(P<0.05), and tightly associated with lymph node metastasis(P<0.05). Though the level of ORAOV1 overexpression was not significant in TSCC, ORAOV1 overexpression level was positively related to histological grade(P<0.05). Both the expression and overexpression of ERCC1 in TSCC were markly lower than those in the normal tissues(P<0.05), and showed a close correlation with lymph node metastasis(P<0.05); and the level of ERCC1 overexpression in the poor differentiation squamous cell carcinoma was higher than that in the well and moderate differentiation squamous cell carcinoma, but the difference was not significant. Conclusion: CCND1 and ORAOV1 were overexpressed in TSCC tissues while ERCC1 was significantly downregulated. The detection of CCND1, ORAOV1 and ERCC1 might play an important role in the early diagnosis of TSCC.