纤维连接蛋白修饰的微沟槽钛片对人牙龈成纤维细胞活性影响的研究

目的:研究微沟槽钛片表面经三氟代乙烷磺酰氯激活并修饰纤维粘结蛋白(fibronectin,FN)后对人牙龈成纤维细胞(HGF)活性的影响。方法:将FN修饰于经过激活的微沟槽钛表面,XPS分析材料表面元素组成;使用cck8法检测HGF接种材料表面6 h、12 h、1 d、3 d、5 d、7 d后的细胞增值率;荧光染色观察接种3 d时的细胞数量及形态。I型胶原纤维ELISA实验及Real-time PCR检测接种5 d时材料表面对HGF分泌功能的差异。结果:在实验观察的6个时间段实验组材料细胞增值率高于对照组(P<0.05)。3 d时HGF在实验组材料表面的伸展率高于空白组(P<0.05)。5 d时FN修饰的两组材料上的细胞外I性胶原纤维的数量及mRNA的表达水平较空白组高(P<0.05)。结论:经三氟代乙烷磺酰氯处理修饰FN的微沟槽钛片可促进HGF的增殖、伸展及分泌功能。

Effectes of Fibronectin-immobilized Microgroove Titanium Plate on Human Gingival Fibroblasts.

Objective: To study the effects of fibronectin (FN)-immobilized microgroove titanium plate on human gingival fibroblasts (HGF). Methods: FN was immobilized to the microgroove of titanium plate after treated with Tresyl chloride. XPS was used to analyze the surface elements. CCK8 was used to evaluate the cell proliferation of HGFs at 6h, 12h, 1d, 3d, 5d and 7d after treated. The ELISA and real-time PCR were used to detect the secretion and expression of collagen I in HGFs at 5d. Results: All the cell number in the experimental group was higher than control group at the predetermined time (P<0.05). The stretch rate of HGFs in the experimental group was higher than control group at 3d (P<0.05). The secretion and mRNA level of collagen I in the experimental group were higher than those of control group at 5d (P<0.05). Conclusion: The FN-immobilized microgroove titanium plate could promote the proliferation, adhesion and secretion function of HGFs.