miRNA－23a在扩张型心肌病中表达的意义

目的观察miR-23a在扩张型心肌病(DCM)外周血中的表达差异,评估其临床意义。 方法收集26例DCM患者作为病例组,收集30例健康者作为对照组。比较两组的临床资料和心脏超声结果,检测脑尿钠肽(BNP)等生化指标,用Real-time PCR检测和比较两组的miR-23a表达水平的差异,并通过ROC曲线分析评估miR-23a对DCM患者的识别能力。 结果DCM组的BNP(605.00±231.71,26.29±17.88,t＝218.61,P<0.05)、左室舒张末直径(LVEDD)高于对照组(64.73±5.74,45.58±2.17,t＝6.09,P<0.05),左室射血分数(LVEF)低于对照组(35.33±4.31,58.72±3.35,t＝－13.54,P<0.05)。miR-23a在DCM组中的表达量高于对照组(3.56±2.56,1.18±0.86,t＝2.85,P<0.01),差异有统计学意义。ROC曲线分析发现,miR-23a对DCM患者具有一定的临床识别能力[AUC＝0.781,95% CI(0.651,0.911),P<0.01]。 结论miR-23a在DCM患者外周血中的表达明显升高,miR-23a可能在DCM患者中具有一定的临床诊断价值。

Expression and significance of miRNA-23a in patients with dilated cardiomyopathy

ObjectiveTo observe the expression of miR-23a in dilated cardiomyopathy (DCM) of the peripheral blood and assess its clinical significance. MethodsTwenty-six patients with dilated cardiomyopathy and 30 healthy controls were enrolled.The clinical data of the two groups, cardiac ultrasound, plasma level of brain natriuretic peptide (BNP) and other biochemical indicators were collected respectively.Real-time polymerase chain reaction (PCR) was used to detect and compare two groups′s miR-23a expression level differences, ROC curve analysis was used to assess miR-23a recognition in patients with DCM. ResultsBNP (605.00±231.71, 26.29±17.88, t=218.61, P<0.05) and left ventricular end-diastdic diameter (LVEDD) (64.73±5.74, 45.58±2.17, t=6.09, P<0.05) in DCM group were higher than those in control group, left ventricular ejection fraction (LVEF) lower than that in control group (35.33±4.31, 58.72±3.35, t=-13.54, P<0.05). miR-23a expression in the DCM group was higher than that in control group (33.56±2.56, 1.18±0.86, t=2.85, P<0.01), the difference was statistically significant.ROC curve analysis demonstrated that miR-23a had certain clinical recognition ability in patients with DCM [AUC=0.781, 95% CI(0.651, 0.911), P<0.01]. ConclusionThe expression of miR-23a elevated in the peripheral blood of patients with DCM, miR-23a may have certain clinical diagnostic value in patients with DCM.