瓜蒂催吐作用有效部位的初步筛选

目的：分离并初步筛选瓜蒂催吐作用的有效部位。方法：以80%乙醇回流提取制得的瓜蒂总提取物后，以用硅胶柱溶剂极性依次递增分离法（石油醚-乙酸乙酯-甲醇连续洗脱）制得的分段提取物（石油醚I，乙酸乙酯II，甲醇III）为药效研究对象，筛选对犬致吐作用有活性的有效部位，最后通过特殊颜色反应确定有效部位化学性质。结果：瓜蒂中乙酸乙酯提取物具有催吐作用，与蒸馏水组相比有显著性差异（P<0.01）。颜色反应说明该部位主要含有葫芦素类等物质。结论：瓜蒂总葫芦素类部位可能是其催吐作用有效部位。

Screening the Emetic Fractions of the Muskmelon Fruit Pedicel

Objective: To separate and screen the effective fractions of the muskmelon fruit pedicel. Methods: With 80% ethanol extract obtained after the muskmelon fruit pedicel total extract by silica gel column in ascending order of solvent polarity separation (petroleum ether - ethyl acetate - methanol continuous elution) obtained sub-extract (petroleum ether I, ethyl acetate II, methanol III) to study the effective part capable of inducing vomiting in dogs. Special color reaction was applied to confirm the chemical property of the effective fractions of the muskmelon fruit pedicel by. Results：The ethyl acetate extract of the muskmelon fruit pedicel has the emetic effects compared with the control group (P<0.01). The effective fractions contained Cucurbitacin substances identified by special color reaction. Conclusions：The effective fractions contained. Cucurbitacin substances might be the emetic fraction in the muskmelon fruit pedicel.