温湿度对高通量酶联免疫吸附实验测定丙型肝炎病毒抗体结果的影响

目的探讨实验室温湿度对高通量酶联免疫吸附实验(ELISA)测定丙型肝炎病毒抗体(HCV-Ab)标准品结果的影响。 方法利用艾德康全自动酶免仪(ELISA 1100型)，恒温24℃、不同相对湿度(%RH)(30%RH、40%RH，50%RH、60%RH、70%RH、80%RH、90%RH)条件下及湿度50%RH、不同温度(16℃、18℃、20℃、22℃、24℃、26℃、28℃、30℃、32℃、34℃、36℃、38℃、40℃)条件下，分别对HCV-Ab标准品进行测定。 结果在排除湿度影响的情况，在温度16℃～36℃条件下进行实验显示，HCV-Ab的S/CO值(分别为3.93±0.09，4.43±0.15，5.16±0.11，6.00±0.17，6.43±0.12，7.43±0.10，7.84±0.15，7.97±0.47，8.24±0.29，8.64±0.61，9.24±0.43)之间差异有统计学意义(F＝620.08，P＝0.000)，且标准品S/CO值均随着温度升高而逐渐升高，HCV-Ab标准品的ELISA结果与温度呈正相关(r＝0.97)。36℃和38℃之间S/CO值(9.24±0.43，9.29±0.30)差异无统计学意义(t＝0.52，P>0.05)，而38℃和40℃之间的S/CO值(9.29±0.30，8.65±0.77)差异有统计学意义(t＝8.17，P<0.05)。而在排除温度影响的情况，不同湿度(30%RH、40%RH，50%RH、60%RH、70%RH、80%RH、90%RH)间S/CO值结果(分别为7.20±0.18，7.09±0.32，5.83±0.27，5.38±0.24，3.91±0.28，3.27±0.49，3.04±0.85)差异有统计学意义(F＝985.64，P＝0.000)，且标准品S/CO值随着湿度升高而逐渐降低，HCV-Ab标准品ELISA结果与湿度呈负相关(r＝－0.98)。 结论不同的温湿度对高通量ELISA检测结果有着重要影响，尽量选择温度不超过36℃和湿度较低的环境进行高通量的ELISA测定，综合考虑推荐全自动酶免仪工作温度范围控制在18℃～25℃之间，湿度控制在35%RH～50%RH之间。

The influence of room temperature and humidity on the measurement results of hepatitis C virus antibody using high-throughput ELISA method

ObjectiveTo explore the influence of room temperature and humidity on the measurement results of hepatitis C virus antibody using high-throughput ELISA method. MethodsTo detect the S/CO values of HCV antibody standard using addcare automatic enzyme immunoassay instrument (ELISA 1100) under 24℃ and different humidity (30%RH, 40%RH, 50%RH, 60%RH, 70%RH, 80%RH, 90%RH) and 50%RH and different temperatures (16℃, 18℃, 20℃, 22℃, 24℃, 26℃, 28℃, 30℃, 32℃, 34℃, 36℃, 38℃, 40℃) respectively. ResultsThe S/CO values of HCV-Ab standard showed significant difference under different temperature (16℃, 18℃, 20℃, 22℃, 24℃, 26℃, 28℃, 30℃, 32℃, 34℃, 36℃), and the S/CO value of standard was positively correlated with temperature.The S/CO value altered little between 36℃ and 38℃, but there was of significant difference between 38℃and 40℃.While the S/CO value of the standard gradually decreased with the rising of humidity(30%RH, 40%RH, 50%RH, 60%RH, 70%RH, 80%RH, 90%RH), which presented significant difference between different humidity (P<0.05), and S/CO value of the standard had a negative correlation with the humidity. ConclusionsRoom temperature and humidity have important effects on high-throughput ELISA detection results.High-throughput ELISA should been carried out in the environment with no more than 36℃ and low humidity.Comprehensive consideration is recommended for the automatic enzyme immunoassay instrument with operating temperature range from 18 to 25 degrees, humidity control between 35%RH~50%RH.