4-1BBL在口腔鳞癌中的表达及临床意义

目的:研究4-1BBL在口腔鳞癌组织和炎症组织中的表达特征。方法:PCR、Real-time PCR、免疫组化检测4-1BBL mRNA和蛋白在口腔鳞癌组织、炎症组织和正常黏膜组织中的表达。结果:PCR 结果显示4-1BBL在口腔鳞癌组织、炎症组织和正常黏膜组织中均有表达,并且Real-time PCR结果显示:与正常组织组比较,口腔鳞癌组4-1BBL mRNA表达量为其2.2106倍,(P<0.05);炎症组织为其1.1151倍,也高于正常组织组(P<0.05);在不同分化期的口腔鳞癌中,高分化组是中分化组的1.516倍(P<0.05),同时也显著高于低分化组(P<0.05)。免疫组化结果:与正常组织比较,鳞癌组织4-1BBL表达显著增高;炎症组织4-1BBL表达介于正常组和鳞癌组之间。同时4-1BBL在不同分化程度的鳞癌组织中,其表达随分化程度的增高而增强。结论:口腔鳞癌组织4-1BBL mRNA和蛋白表达显著高于正常组织和炎症组织;且其表达随分化程度的升高而增强。

Expression and Clinical Significance of 4-1BBL in Oral Squamous Cell Carcinomas.

Objective: To study the differences of 4-1BBL expression between in oral squamous cell carcinomas and inflammation. Methods: The expression of 4-1BBL in oral squamous cell carcinomas, inflammation tissues and normal tissues were detected by PCR , Real-time PCR and immunohistochemistry. Results: The results of PCR showed that 4-1BBL in oral squamous cell carcinomas, inflammation tissues and normal tissues all had expressed. In addition, compared with normal tissues, the 4-1BBL mRNA expression in oral squamous cell carcinomas increased to 2.2106 fold (P<0.05); the expression in inflammation tissues imcreased to 1.1151 fold (P<0.05); In different differentiation stages of oral squamous cell carcinomas, it was 1.516 times of high differentiation group than moderately differentiation group (P<0.05), but also significantly higher than in poorly differentiated group (P<0.05). Immunohistochemical Results: compared with normal group, the expression of 4-1BBL was significantly more higher in squamous carcinoma tissues; the expression of 4-1BBL in inflammation tissue was between normal group and squamous cell carcinomas. At the same time the different differentiated squamous carcinoma tissues enhanced by the increasing of the degree of differentiation. Conclusion: The expression of 4-1BBL mRNA and protein in the oral squamous carcinoma tissues is significantly higher than normal and inflammation tissues; And its expression increases with the degree of differentiation.