siRNA干扰对变形链球菌耐氟菌ffh基因产酸能力的影响

目的:探讨变形链球菌耐氟菌(UA159-FR)ffh基因 siRNA干扰后对产酸能力的影响。方法:电穿孔法将siRNA转入UA159-FR与ffh基因序列靶向位点结合,将干扰前后细菌分别加入BHI培养液,含5%糖类的葡萄糖、蔗糖、乳糖、淀粉中培养,pH调至7.5,24 h后测终末pH。结果:转染结果成功;干扰前后不同糖培养基中变形链球菌耐氟菌ffh基因对产酸有显著差异(P<0.05);干扰前后在常规、葡萄糖、及乳糖中差异极显著(P<0.01);蔗糖中差异显著(P<0.05);淀粉中无显著差异。结论:变形链球菌耐氟菌中基因ffh对产酸能力影响显著。

Effect of siRNA Interference on the Acidogenicity for Gene ffh of Fluoride Resistant Streptococcus Mutans.

Objective: To have a discussion on the effect on the acidogenicity for gene ffh of fluoride resistant streptococcus mutans (UA159-FR)after siRNA interference. Methods: UA159-FR was transformed by using electroporation and combined with targeted site of ffh gene sequence, and cultured by adding BHI, glucose, saccharose, lactose and starch with 5% saccharides into bacteria that was before interfered and after interfered respectively. PH was set to 7.5. The terminal PH was measured after 24 hours. Results: After the transfection, the result turned out to be successful. Before and after interference, there were some significant differences for transfection of fluoride resistant streptococcus mutans among different culture media of gene ffh (P<0.05).△PH of normal, glucose and lactose were significantly different before and after the interference(P<0.01); It was significant in sucrose(P<0.05) and there was no obvious difference in starch. Conclusion: The effect of siRNA interference on the acidogenicity for gene ffh of fluoride resistant streptococcus mutans is significant.