煅烧骨复合PRF对牙周骨缺损的实验研究

目的:研究比较异种煅烧骨和Bio-oss骨分别与富血小板纤维蛋白(platdet-rich fibrin,PRF)联合,及其各自单独使用对牙周骨缺损修复的效果。方法:制备煅烧骨,PRF凝胶及其复合物。选54只新西兰大白兔,制备牙周组织缺损模型,随机分成空白对照组、PRF凝胶组、煅烧骨组、Bio-oss骨组、煅烧骨/PRF凝胶组、Bio-oss骨/PRF凝胶组,将这些材料植入牙周缺损区,每组9只兔子。术后4周、8周和l2周处死动物,获取完整标本,观察影像学和组织学。行组织学染色检测,测量新生牙周组织的高度。结果:X线结果表明:4周、8周和l2周煅烧骨/PRF 组和Bio-oss/PRF 组平均密度均明显高于对照组且缺损处骨组织连续。组织学观察:实验组均在牙根面可见新生牙骨质样组织、牙周膜和牙槽骨样组织的形成,随着时间的推移逐渐成熟、增多。12周时,煅烧骨/PRF凝胶、Bio-oss骨/PRF凝胶较空白对照组明显提高,较其他3组差异有统计学意义(P<0.05)。结论:煅烧骨/PRF凝胶、Bio-oss骨/PRF凝胶复合物可明显促进牙周组织的再生。

Empirical Study of Reparation of Periodontal Bone Defects Treated with PRP and Calcined Bone Composite.

Objective: To study the dissimilar calcined bone and Bio-oss bone respectively, and composite with platelet rich fibrin (platdet-rich fibrin, PRF), and their respective alone on the effect of periodontal bone defects. Methods: Calcined bone, PRF gel and complex were prepared. 54 rabbits were selected. Periodontal tissue defect model was randomly divided into control group, PRF gel group, calcined bone group, Bio-oss bone group, calcined bone/PRF group, Bio-oss bone/PRF group. After four weeks, eight weeks and l2 weeks the animals were sacrificed for a complete specimen, radiological and histological observation. Histological staining was used to measure the height of newborn periodontal tissues. Results: X-ray results showed that: 4 weeks, 8 weeks and l2 weeks calcined bone/PRF group and Bio-oss/PRF group were significantly higher than the average density, and bone tissue defects continuously. The experimental group was seen in root surfaces like tissue formation of new cementum , periodontal ligament and alveolar bone -like tissue matures over time, increasing. 12 weeks , the calcined bone/PRF, Bio-oss bone/PRF significantly improved compared with the control group, compared with the other three groups were statistically significant (P<0.05). Conclusion: Calcined bone/PRF gel and Bio-oss bone/PRF gel complexes can significantly promote the regeneration of periodontal tissues.