饥饿状态下白色念珠菌差异表达基因的研究

目的探讨白色念珠菌饥饿状态的形成机制及差异表达基因。 方法将白色念珠菌（ATCC 10231）诱导进入厌氧饥饿状态，收集进入饥饿状态0、12、24和48 h的白色念珠菌，提取总RNA后采用全基因组表达谱芯片技术分析基因的差异表达。采用t检验对不同时间点的基因表达状况进行统计分析。 结果统计分析显示，白色念珠菌诱导进入饥饿状态过程中，14.45%~ 29.13%的基因表达发生改变（P<0.05），涉及氨基酸代谢、糖代谢、脂类代谢、核酸代谢和细胞周期等多个通路。上述通路中丙氨酸合成通路、ATP合成基因SDH4、有丝分裂相关基因PRE1以及胞外基质分泌相关基因GCA1等表达降低，RAX2、VPS34等耐药相关基因表达升高。 结论白色念珠菌在诱导进入饥饿状态过程中涉及代谢和增殖水平的基因表达下降，对外界刺激和药物耐受相关基因表达上调，此类改变有助于保持白色念珠菌在饥饿状态的存活力和致病性。

Differentially expressed genes of Candida albicans under starvation conditions

ObjectiveTo explore the gene expression during induction and formation of starvation state of Candida albicans (C.albicans) . MethodsC.albicans American Type Culture Collection (ATCC) 10231 was cultured and induced into starvation phase. After induction for 0, 12, 24, and 48 h, the fungal cells were collected. Total RNA was extracted and microarrays were used to identify the gene expression changes under starvation conditions. T-test was used to statistically analyze the data of different time points. ResultsStatistical analysis demonstrated that 14.45% to 29.13% of gene expression has changed during the induction into starvation conditions (P<0.05) , these genes are related with many biologic pathways including amino acid metabolism, carbohydrate metabolism, lipid metabolism, nucleic acid metabolism and cell cycles. Especially, the differentially expressed genes included the down-regulation of alanine metabolism related genes, ATP synthesis related gene SDH4, mitosis related gene PRE1, and exopolymeric matrix production gene GCA1, and up-regulation of PXA2 and VPS34. ConclusionsDuring the induction into starvation conditions, the genes related with metabolic and proliferative activities were down-regulated, while the genes related with environment and drug resistance were up-regulated. These changes may be contributed to maintain the survival and pathogenic capabilities of C.albicans under starvation conditions.