Ptch和Smo在舌鳞状细胞癌Tca8113细胞中的表达及其意义

目的:研究Hedgehog(Hh)信号通路相关因子Ptch和Smo在舌鳞状细胞癌Tca8113细胞株中的表达及其意义。方法:Hh信号通路抑制剂Cyclopamine处理Tca8113细胞后,采用MTT实验检测细胞增殖抑制率,再利用RT-PCR法和Western-Blot方法检测 Ptch和Smo的mRNA和蛋白表达情况。结果:Cyclopamine对Tca8113细胞增殖具有抑制作用。加药后的不同时间段中,显示Ptch、Smo的mRNA和蛋白在Cyclopamine药物组中的表达低于对照组。Smo的mRNA和蛋白分别在加药后24 h、72 h中的表达随着药物浓度增高而降低。结论:Cyclopamine可以抑制Tca8113细胞的生长,降低Smo mRNA和蛋白的表达,Hh信号通路可能在舌癌的发生、发展中起重要作用。[关键词] Hh信号通路 Tca8113细胞 Cyclopamine Ptch Smo

Eexpression and Significance of Ptch and Smo in the Tongue Cancinoma Tca8113 Cell lines.

Objective: To investigate the expression and significance of the hedgehog signaling pathway factors Ptch and Smo in the tongue cancinoma Tca8113 cell lines. Methods: Aftter treatment of Tca8113 cells with hedgehog signaling pathway antagonist Cyclopamine, the proliferation-inhibition rate was detected by MTT assay. And mRNA and protein levels of Ptch and Smo were analyzed by RT-PCR and Western blot, respectively. Results: Cyclopamine inhibited Tca8113 cells. In the different times treatment of Tca8113 cells with Cyclopamine, the expression of Ptch and Smo mRNA in the Cyclopamine groups was lower than the control group, and the protein expression was the same with mRNA. In the 24h treatment of Tca8113 cells with Cyclopamine, the expression of Smo mRNA in the Cyclopamine groups was in dose-dependent manner, and in 72h the expression of Smo protein in the Cyclopamine groups was in dose-dependent manner. Conclusion: Cyclopamin specifically inhibited the Tca8113 cells migration and inhibited the expression of Smo mRNA and protein. The Hh signaling might be involved in the carcinogenesis and progression of tongue cancer.