CD133在口腔扁平苔藓和口腔鳞状细胞癌中的表达及其临床意义

目的探究肿瘤干细胞标记物CD133在口腔正常黏膜、口腔扁平苔藓（OLP）及口腔鳞状细胞癌（OSCC）中的表达情况及临床意义，评估其作为OLP恶性转化的早期诊断指标、OSCC治疗干预靶标的临床价值，为进一步研究口腔黏膜癌变机制提供基础。 方法回顾性分析10例正常口腔黏膜、60例OLP、60例OSCC患者的临床资料，运用免疫组织化学技术检测各组病理组织中CD133表达情况，采用Mann-Whiney秩和检验比较各组间CD133表达差异，卡方检验统计分析CD133与各临床因素的关系。采用免疫组织化学技术和免疫印迹技术检测人口腔癌前病变细胞株（DOK）和人OSCC细胞株（CAL-27）中CD133的表达情况，t检验比较CD133在DOK与CAL-27细胞株中含量差异。 结果口腔正常黏膜、OLP、OSCC三组中，CD133的阳性率为0（0/10）、31.67%（19/60）、63.33%（38/60），表达逐渐增强。CD133在口腔正常黏膜与OLP表达强度差异有统计学意义（Z=-2.046，P= 0.041）。CD133在OLP与OSCC表达强度差异具有统计学意义（Z=-3.777，P<0.001）。CD133在DOK中弱阳性表达，在CAL-27中阳性表达，DOK与CAL-27中CD133含量差异有统计学意义（t=-5.029，P= 0.001）CD133与OSCC的临床分期和淋巴结转移有关。 结论CD133作为评估OLP恶性转化潜能的指标及OSCC早期治疗干预靶标可能具有重要临床价值。

The clinical significance of the expression of CD133 in oral lichen planus and oral squamous cell carcinoma

ObjectiveTo investigate the expression of CD133 in oral normal mucosa, oral lichen planus (OLP) and oral squamous cell carcinoma (OSCC) , to analyze the relationship between its expression and the clinical factors, and to evaluate the significnce of CD133 as an index to evaluate the canceration potency of OLP. MethodsImmunohistochemical analysis and Mann-Whiney test were applied to investigate the expression of CD133 in paraffin section of human oral normal mucosa, OLP and OSCC tissues. Then the relationships between its expression and clinical factors were analyzed. Immunohistochemical analysis, Western blot analysis and t test were also applied to investigate the expression of CD133 in human dysplastic oral keratinocyte (DOK) cell line and human oral squamous cell carcinoma (CAL-27) cell line. ResultsA gradual increase in the expression of CD133 was observed in three groups, the positive rates of CD133 were 0 (0/10) , 31.67% (19/60) , 63.33% (38/60) respectively. Significant differences were observed in expression of CD133 in oral normal mucosa and OLP (Z=-2.046, P= 0.041) , OLP and OSCC (Z=-3.777, P<0.001) . An increase in the expression of CD133 were also observed from DOK cell line to CAL-27 cell line (t=-5.029, P= 0.001) . ConclusionCD133 can be used as clinical index to evaluate the canceration potency of oral lichen planus.