低氧增强自噬促进舌鳞状细胞癌细胞迁移和侵袭

目的探讨低氧条件下对舌鳞状细胞癌（TSCC）自噬活性和迁移能力的影响。 方法应用含1% O₂的低氧培养箱培养TSCC UM1细胞，Western blot检测低氧诱导3、6、9、12、24 h后低氧诱导因子1α（HIF-1α）和自噬标记蛋白Beclin-1、自噬相关蛋白5（ATG5）和微管相关蛋白轻链3（LC3）的表达变化；细胞划痕实验检测低氧诱导后细胞迁移能力的改变；Transwell侵袭小室实验检测低氧诱导后细胞的侵袭能力；SPSS 13.0统计软件进行数据分析。 结果低氧条件下，与对照组（0.527 ± 0.055）相比，TSCC UM1细胞自噬体双层膜标记蛋白LC3Ⅱ的表达（1.206 ± 0.053）增加（t= 12.96，P<0.001），同时自噬相关蛋白Beclin-1表达量（1.151 ± 0.078）较对照组（0.775 ± 0.062）明显提高（t= 6.736，P= 0.001），ATG5的表达（1.231 ± 0.06）较对照组（0.711 ± 0.052）也明显上升（t= 7.834，P= 0.001）。与对照组相比，低氧诱导后细胞迁移能力（0.349 ± 0.024）较对照组（0.788 ± 0.037）明显增加（t= 9.918，P= 0.001），细胞侵袭能力（23 ± 2）较对照组（71 ± 4）明显增强（t= 9.528，P= 0.001）。 结论低氧条件下可以增强TSCC细胞自噬活性，促进其迁移和侵袭。

Hypoxia induced autophagy and promotes the migration and invasion in tongue squamous cell carcinoma

ObjectiveTo investigate the effect of hypoxia on autophagy and migration of tongue squamous cell carcinoma (TSCC) cell line. MethodsUM1 cells were cultured in hypoxia incubator containing 1% oxygen concentration. Western blot was performed to examine the expression of HIF-1α, Beclin-1, ATG5 and LC3 following 3, 6, 9, 12 and 24 h of treatment of hypoxia. Wounding healing was used to evaluate cell migration. Transwell assay was carried out to assess cell invasion. Statistical analysis was performed using SPSS 13.0 software. ResultsAfter, The expression level of autophagic protein LC3Ⅱ (1.206 ± 0.053) in the hypoxia treatment group was higher than control group (0.527 ± 0.055) and both showed statistically significant difference (t= 12.96, P<0.001) ; the expression level of Beclin-1 (1.151 ± 0.078) in the hypoxia treatment group was higher than control group (0.775 ± 0.062) , and both showed a statistically significant difference (t= 6.736, P= 0.001) ; the expression level of ATG5 (1.231 ± 0.06) in the hypoxia treatment group was higher than control group (0.711 ± 0.052) , was and both showed a statistically significant difference (t= 7.834, P= 0.001) . The migration ability of TSCCUM1 (0.349 ± 0.024) was improved under hypoxia in comparison to control groups (0.788 ± 0.037) (t= 9.918, P= 0.001) . The invasion ability of TSCCUM1 (23 ± 2) was upregulated under hypoxia in comparison to control groups (71 ± 4) (t= 9.528, P= 0.001) . ConclusionHypoxia can improve autophagic activity levels and promotes the migration and invasion in tongue squamous cell carcinoma cell line.