山东省HIV-1流行毒株的基因特征和系统树分析

从13名山东省HIV感染者的淋巴细胞(PBMC)中提取前病毒DNA,使用套式PCR方法扩增HIV-lenv基因的C2-V3区。经序列分析发现13份样品均为B′,亚型(泰国B亚型),其与国际参考序列Bcon、泰国代表株93th067A、中国云南省代表株RL42、云南省共享序列96Yn-Bcon的基因离散率分别为7.31％、5.72％、4.25％、3.76％,而远离其它亚型。由系统树可以更清楚看到13个毒株全部与93th067A、RL42等B,亚型流行株聚在一起。通过GCG软件包计算这些毒株彼此间的基因离散率的变异系数为2.71％。以上数据提示HIV-1在山东省的流行时间不长。且与云南省相同亚型的HIV-1毒株密切相关。

Genetic Characterization and Phylogenetic Analysis of HIV-1 Strains in Shandong province

The provirus DNA were extracted from the peripheral blood mononuclear cell (PBMCs) in HIV-1 infective individuals of Shandong province, and C2-V3 of the env gene were amplified by nested-PCR and sequenced directly. Sequence analysis showed that all of them were subtype HIV-1 B strains(Thailand B). The average genetic distances between the 13 strains and the international reference strains were 7.31%(Bcon, international consensus sequence), 5.72%(93th067A, Thailand strains), 4.25%(RL42, Yunnan strain in China), 3.76%(96Yn-Bcon, Yunnan consensus sequence), respectively. On the phylogenetic tree, all of them clustered with B subtype strains 93th067A and RL42. The genetic divergence innergroup was 2.71%. The above data suggests that the time of recent epidemic of HIV-1 in Shandong be not long and responsible virus very close to those identified in Yunnan province.