丹参注射液对庆大霉素耳蜗毒性的防护

背景丹参可有效减轻卡那霉素对CBA小鼠的耳毒性效应;然而,其是否也能防护庆大霉素对豚鼠的耳蜗毒性,目前尚不明确.目的研究丹参注射液对庆大霉素耳蜗毒性的防护作用.设计完全随机对照的实验研究.地点和对象实验在中国医科大学听力研究室进行.选用清洁级健康纯白红目豚鼠40只,雌雄不限,体质量250～300 g,耳郭反射灵敏,鼓膜及外耳道正常,由中国医科大学第二医院动物中心提供.干预动物随机分成4组,即对照组、庆大霉素组、庆大霉素+丹参注射液组和丹参注射液组,每组各10只.庆大霉素组每日腹腔注射硫酸庆大霉素100 mg/kg;丹参注射液组每日腹腔注射丹参注射液6 g/kg;庆大霉素+丹参注射液组同时注射硫酸庆大霉素100 mg/kg和丹参注射液6 g/kg;对照组则每日腹腔注射等量生理盐水.4组皆连续用药10 d.用药期间每天监测体质量以调整药量.全部实验由作者和另一名硕士研究生完成.主要观察指标应用听脑干反应(auditory brainstem response,ABR)测试,观察用药前后听阈变化.应用透射及扫描电镜技术,观察豚鼠耳蜗形态学变化.结果用药10d后,庆大霉素组ABR阈值(36.00±5.48)dB SPL明显升高,与对照组(17.00±4.47)dB SPL比较差异有显著性意义(t=6.008,P＜0.01);庆大霉素+丹参注射液组ABR阈值(27.00±4.47)dB SPL较庆大霉素组(36.00±5.48)dB SPL明显降低,差异有显著性意义(t=2.846,P＜0.05).电镜观察显示庆大霉素+丹参注射液组耳蜗结构损害明显轻于庆大霉素组.结论丹参注射液能有效防护庆大霉素的耳蜗毒性.

Protective effect of injectio Salvia Miltiorrhiza on gentamicin cochleotoxicity

BACKGROUND: Radix Salvia Miltiorrhiza can effectively attenuate Kanamycin-induced hearing loss in CBA mice. However, whether it can also prevent gentamicin(GM) -induced cochleotoxicity in guinea pigs is still unclear.OBJECTIVE: To investigate the protective effect of injectio Salvia Miltiorrhiza(SM)on GM-induced cochleotoxicity.DESIGN: A completely randomized control experimental study.SETTING and PARTICIPANTS: The study was performed in Hearing Research Laboratory of China Medical University. Forty healthy albino guinea pigs of either sex, cleaning grade, with body mass of 250 to 300 g, with sensitive Preyer' s reflexes and normal drum membrane and external auditory canal, were provided by Animal Center of Second Hospital of China Medical University.INTERVENTIONS: Animals were randomly assigned to 4 groups( n = 10):Control group, GM group, GM + SM group, and SM group. GM group, received gentamicin sulfate 100 mg/kg daily by intraperitoneal injections; SMgroup, received injectio Salvia Miltiorrhiza 6 g/kg daily by intraperitoneal injections; GM + SM group, received gentamicin sulfate 100 mg/kg and injectio Salvia Miltiorrhiza 6 g/kg by intraperitoneal injections simultaneously;Control group, received the equivalent of sterile saline by intraperitoneal injections. Duration of treatment was 10 days for all groups. Body mass was monitored daily, and the administered dose was adjusted accordingly. Author and another master accomplished all experiments.MAIN OUTCOME MEASURES: Auditory bralnstem response(ABR) measurement was used to examine hearing thresholds. Transmission and scanning electron microscopic technique were conducted to detect cochlear morphology.RESULTS: After 10 days of drug injection, ABR thresholds in GM group (36.00 ± 5.48) dB SPL]were significantly higher than those in the control group ( 17.00 ± 4.47 ) dB SPL], with a significant difference between GM and control group(t =6.008, P ＜ 0.01); ABR thresholds in GM+SM group (27.00± 4.47) dB SPL]were significantly lower compared with those in GM group (36. 00 ± 5.48) dB SPL], with a significant difference between GM + SM and GM group( t = 2. 846, P ＜ 0.05 ). Under electron microscope, damage of cochlear structure in GM + SM group was significant alleviated compared with that in GM group.CONCLUSION: SM can effectively reduce GM-induced cochleotoxicity.