T—AK细胞上清液抑瘤活性的研究

本文应用ＭＴＴ比色法检测了ｒＩＬ－２和抗ＣＤ３单抗共同诱导激活的Ｔ－ＡＫ细胞培养上清液对Ｋ５６２细胞和ＢＥＬ－７４０２细胞增殖的抑制效应，并观察了Ｔ－ＡＫ上清液抑瘤活性与Ｔ－ＡＫ细胞杀伤活性的相关性，结果表明：（１）Ｔ－ＡＫ细胞能够自发分泌一些抑制Ｋ５６２细胞和ＢＥＬ－７４０２细胞增殖的抑制因子，但活性很低；（２）Ｔ－ＡＫ细胞在杀伤Ｋ５６２细胞过程中释放的抑制肿瘤细胞生长的物质，抑瘤活性比较高，共

Study on Antitumor Activities in Supernatant Preparations from T-AK Cells in Culture

The antitumor effects of supernatant preparation, made from the culture media of T-activited killer(T-AK)cells costimulated with interleukin-2 (rIL-2)and anti-CD3 monoclonal antibody (CD3McAb) , on K562 (leukemia ) and BEL-7402 cells (hepatic carcinoma ) were studied, and the interrelationship between the cytolytic activity of T-AK cells and the tumor-inhibitory activity of T-AK supernatant was observed. It was found that (1). T-AK cells produced spontaneously some inhibitory factors which could inhibite the proliferation of K562 cells and BEL-7402 cells ,but the inhibitory activity was in a low level. (2) The tumor-inhibitory substances released from the process of T-AK cells killing K562 cells had a higher and nonspecific cytotoxicity on the tumor cells. (3). The cytolytic activity of T-AK cells had a positive interrelationship with the tumor-inhibitor activity of T-AK supernatant preparation. These results showed that the indirect cytotoxicity of inhibitory factors in T-AK supernatant was one of the antitumor mechanisms of T-AK cells.