反相高效液相色谱法测定人血浆中洛沙平浓度

目的建立测定人血浆中洛沙平浓度的反相高效液相色谱法。方法以DiamonsilC18反相柱（150mm×4．6mm，5μm）为色谱柱，流动相为0．03mol·L^-1磷酸二氢钾（调pH6．4）-乙腈-83：17；流速：1．0mL·min^-1；柱温：40℃；检测波长：256nm。以乙酸乙酯为提取剂。结果洛沙平的高、中、低（1000．0、400．0、10．0ng·mL^-1）3个浓度平均回收率分别为99．75％、98．94％、101．93％，日内、日间RSD均〈7％（n=5）；分析方法的最低检测限为3．0ng·mL^-1；线性范围为10．0～1000．0ng·mL^-1。曲线方程：Y=83．52X-3．65，r=0．9995（n=10）。结论该方法灵敏、准确、简单、快速，可用于临床血药浓度监测和药动学研究。

Determination of loxapine in human plasma by RP-HPLC

Objective To establish a method to determine the concentration of loxapine in human plasma by RP-HPLC. Methods The drug from the plasma was analyzed in an RP-HPLC system Diamtnsil C18 column （150 min×4. 6 mm, 5 μm） ; The mobile phase consisted of 0.03 mol·L^-1 postassium dihydrogen phosphate （pH 6.4） -acetonitrile （83 ： 17） ; the flow rate was 1.0 mL ·min^-1 ; the detection wavelength was 256 nm. Ethyl acetate was used as the extracting solvent. Results The average recoveries of loxapine in high, middle and low concentrations （1 000. 0, 400. 0, 10.0 ng·mL^-1） were 99.75%0 , 98.94% and 101.93%, respectively. The intra-day and inter-day variation （RSD） was less than 7% （n=5） . The calibration curve of loxapine showed good linearity, r=0. 999 5 （n= 10）, over the range of 10. 0-1 000.0 ng · mL^-1. The regression equation was Y=83.52X-3.65. r=0. 999 5 （n=10）. The minimum detectable concentration of loxapine was 3.0 ng·mL^-1. Conclusion The method is sensitive, accurate, easy and auick, which can be used for clinical drug monitoring and pharmacokinetic studies.