HPLC测定聚乙二醇修饰科博肽含量

目的测定聚乙二醇修饰科博肽含量。方法采用高效液相色谱法（HPLC），色谱条件为C8色谱柱（4．6mm×250mm，5μm），0．1％三氟醋酸溶液为流动相A，三氟醋酸．80％乙腈（1：1000）为流动相B，流速为1mL·min^-1，检测波长为221nm。结果该法的线性范围为51．6～258μg·mL^-1，r=0．9994，回收率为99．9％，RSD=0．50％（n=9）。结论本法简便、准确、重复性好，可作为聚乙二醇修饰科博肽的含量测定方法。

Determination of PEG-CBT by HPLC

OBJECTIVE To determine the content of PEG-CBT. METHODS The content of PEG-CBT was determined by HPLC with C8 column （4.6 mm×250 mm, 5 μm）. The mobile phase were combined with solution A （0.1% trifluoroacetic acid） and solution B （trifluoroacetic acid-80% acetonitrile （1 ： 1 000）. The flow rate was 1 mL·min^-1, and the detection wavelength was 221 nm. RESULTS The linear range was 51.6-258 μg·mL^-1 （r=0.999 4）. The average recovery rate was 99.9%, and RSD was 0.50%（n=9）. CONCLUSION The method is simple, accurate, and reproducible and can be used for the determination of PEG-CBT.