| 天然红心蛋中类胡萝卜素提纯物对人肝癌细胞增殖和凋亡的影响 |
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| 引用本文： | 刘良忠,张民,黄进,石嘉怿,彭光华,张声华. 天然红心蛋中类胡萝卜素提纯物对人肝癌细胞增殖和凋亡的影响[J]. 营养学报, 2004, 26(6): 457-459 |
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| 作者姓名： | 刘良忠 张民 黄进 石嘉怿 彭光华 张声华 |
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| 作者单位： | 1. 长江大学生命科学学院,434103
2. 华中农业大学食品科技学院,430070 |
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| 基金项目： | 国家博士点基金项目(No.20020504008) |
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| 摘 要： | 目的:探讨天然红心蛋中类胡萝卜素提纯物(carotenoidsextractsfromnaturalredyolk,CENRY)对人肝癌细胞QGY-7703细胞增殖和凋亡的影响。方法:采用体外培养,细胞增殖实验,激光扫描共聚焦显微镜(LSCM)观察细胞形态及钙离子浓度变化,采用流式细胞术检测细胞周期等。结果:CENRY1.0、0.5、0.25μmol/L三个剂量能显著抑制人肝癌QGY-7703细胞增殖,细胞凋亡指数随剂量增加及时间延长而上升;CENRY对QGY-7703细胞存在G2/M期阻滞作用;处理组细胞内Ca2+浓度极显著地升高。结论:CENRY能抑制QGY-7703增殖、诱导凋亡。
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| 关 键 词： | 天然红心蛋中类胡萝卜素提纯物 细胞增殖 细胞凋亡 |
| 文章编号： | 0512-7955(2004)06-0457-03 |
| 修稿时间： | 2003-12-01 |
EFFECTS OF CAROTENOIDS EXTRACTS FROM NATURAL RED YOLK ON PROLIFERATION AND APOPTOSIS OF HUMAN LIVER CANCER CELL |
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| LIU Liang-zhong,ZHANG Min,HUANG Jing,SHI Jia-yi,PENG Guang-hua,ZHANG Sheng-hua. EFFECTS OF CAROTENOIDS EXTRACTS FROM NATURAL RED YOLK ON PROLIFERATION AND APOPTOSIS OF HUMAN LIVER CANCER CELL[J]. Acta Nutrimenta Sinica, 2004, 26(6): 457-459 |
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| Authors: | LIU Liang-zhong ZHANG Min HUANG Jing SHI Jia-yi PENG Guang-hua ZHANG Sheng-hua |
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| Affiliation: | LIU Liang-zhong,ZHANG Min,HUANG Jing,SHI Jia-yi1,PENG Guang-hua,ZHANG Sheng-hua |
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| Abstract: | Objective: To observe the effects of carotenoids extracts from natural red yolk(CENRY) on proliferation and apoptosis of human liver cancer line QGY-7703. Methods: MTT and laser scanning confocal microscope (LSCM) were used to observe cellular morphological change and determine the Ca2 concentration in cells. FCM was used to check cell cycle. Result: After treated with CENRY, the proliferation of QGY-7703 was markedly inhibited. The apoptosis index was increased when the dose of CENRY increased and treatment time prolonged. The cell cycle progression was arrested at G2/M phase. The concentration of intracellular Ca2 was remarkably increased. Conclusion: CENRY can induce the apoptosis and inhibition of the proliferation of QGY-7703 cells . |
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| Keywords: | carotenoids extracts from natural red yolk cell proliferation apoptosis |
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