Characterization of a genomic region encoding the 32-kDa dense granule antigen of Sarcocystis muris (Apicomplexa) |
| |
Authors: | Bernd Freyer Thomas Hansner Heinz Mehlhorn Wolfgang Rüger |
| |
Institution: | Lehrstuhl für Biologie der Mikroorganismen, Arbeitsgruppe Molekulare Genetik, Ruhr-Universit?t Bochum, Universit?tsstra?e 150, D-44780 Bochum, Germany e-mail: wolfgang.rueger@ruhr-uni-bochum.de, Fax: +49234-7094195, DE Institut für Zoomorphologie, Zellbiologie und Parasitologie der Heinrich-Heine Universit?t, Universit?tsstra?e 12, D-40225 Düsseldorf, Germany, DE
|
| |
Abstract: | Two subgenomic libraries constructed from Sarcocystis muris total DNA were screened with a cDNA probe, specific for a 32-kDa protein associated with the dense granules. Two clones reacted
positively and were isolated, gDG 32/1 and gDG 32/2. Genomic clone gDG32/1 and part of clone gDG 32/2 have been sequenced.
The composite nucleotide sequence of these genomic clones comprises 4.34 kb. It contains a 5′ region of 2.14 kb, a first coding
region (222 bp, exon I), a noncoding region (608 bp, intron), a second coding region (660 bp, exon II), and a 3′ region of
693 bp. The upstream region shows a eukaryotic promoter structure and a consensus sequence for the 5′ and 3′ splicing sites.
Thus the open reading frame (ORF DG32) coding for the 32-kDa protein of the dense granules of S. muris cyst merozoites is interrupted by an intron. To our knowledge, dg32 is the first sarcosporidian mosaic gene to be characterized.
Received: 27 April 1999 / Accepted: 11 May 1999 |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|