| 骨髓基质细胞在藻酸盐凝胶中的生物学行为 |
| |
| 引用本文： | 孙磊,胡蕴玉,窦榆生,孟国林,徐建强. 骨髓基质细胞在藻酸盐凝胶中的生物学行为[J]. 中国矫形外科杂志, 2002, 9(2): 158-160 |
| |
| 作者姓名： | 孙磊 胡蕴玉 窦榆生 孟国林 徐建强 |
| |
| 作者单位： | 1. 第四军医大学全军创伤骨科研究所,710032
2. 西安红十字会医院 |
| |
| 基金项目： | 中国博士后科学基金资助 (课题编号 :1 9991 0 ) |
| |
| 摘 要： | 目的：探讨骨髓基质细胞（Bone Marrow Stromal Cells,MSC)在海藻酸盐（Alginate)中的生物学行为，为在组织工程研究中选用海藻酸盐做为骨髓基质细胞的载体提供理论依据。方法：将地塞米松诱导后的骨髓基质细胞种植在1%海藻酸盐中，以HE染色，BMP-2免疫组化染色，扫描电镜观察骨髓基质细胞在凝胶中的生物学行为。结果：凝胶中细胞呈“悬浮”生长状态，可见到细胞分裂和核分裂相；细胞在凝胶中增殖，4d后可以见到基质分泌；海藻酸盐中培养组与常规培养组MSC的BMP-2阳性表达率无显著差异。结论：在海藻酸盐中骨髓基质细胞的生物学行为表现良好。此特征适宜作为骨组织工程的载体。
|
| 关 键 词： | 骨髓基质细胞 海藻酸盐 凝胶 生物学行为 |
| 文章编号： | 1005-8478(2002)02-0158-03 |
| 修稿时间： | 2001-06-06 |
Bioaction of Bone Marrow Stromal Cells in Alginate Gel |
| |
| SUN Lei,HU Yun yu,DOU Yu sheng,et al.. Bioaction of Bone Marrow Stromal Cells in Alginate Gel[J]. The Orthopedic Journal of China, 2002, 9(2): 158-160 |
| |
| Authors: | SUN Lei HU Yun yu DOU Yu sheng et al. |
| |
| Affiliation: | SUN Lei,HU Yun yu,DOU Yu sheng,et al. The Orthopedic and Trauma Institute of PLA,The Fourth Military Medical University,Xi'an 710032 |
| |
| Abstract: | Objective: To appreciate bio action of bone marrow stromal cells(BMSC) in alginate in order to offer a theoretical base using alginate gel as bone tissue engineering carrier. Method: Bone marrow stromal cells induced by Dexamethason were implanted in 1% concentrate alginate, and were stated by method of HE stain, BMP 2 immunity histochemical stain and SEM. Result: Cells living in alginate were `floating', nuclear disintegration and cell cleavage were observed. Proliferation and stromal secretion were seen after 4 day's implanting. Light demanding rate of BMP 2 expression of BMSC had no difference with which cultured normally. Conclusion: Bio action of BMSC in alginate present well. |
| |
| Keywords: | Bone Marrow Stromal Cells(BMSC) Alginate Gel |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
