| 根皮素对rBMECs摄取人参皂苷Rb1的影响 |
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| 引用本文： | 王玉珠,顾圣莹,张吉刚,朱冠华,武鑫,刘皋林,李晓宇. 根皮素对rBMECs摄取人参皂苷Rb1的影响[J]. 中国药学杂志, 2015, 50(3): 226-231. DOI: 10.11669/cpj.2015.03.010 |
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| 作者姓名： | 王玉珠 顾圣莹 张吉刚 朱冠华 武鑫 刘皋林 李晓宇 |
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| 作者单位： | 上海交通大学附属第一人民医院临床药学科, 上海 200080
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| 基金项目： | 科技部十二五重大新药创制专项(2011ZX09302-007-02);上海市自然科学基金资助项目(10ZR1423600);中国博士后科研基金特别资助(200902245) |
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| 摘 要： | 目的 以原代培养的大鼠脑微血管内皮细胞为模型,研究根皮素对大鼠脑微血管内皮细胞摄取人参皂苷Rb1的影响。方法 实验分为2组,人参皂苷Rb1对照组和根皮素干预组。运用LC-MS/MS测定大鼠脑微血管内皮细胞对人参皂苷Rb1的摄取量,BCA法测细胞蛋白浓度。结果 根皮素干预组大鼠脑微血管内皮细胞对人参皂苷Rb1的摄取量显著低于人参皂苷Rb1对照组(P<0.05)。结论 根皮素可显著抑制大鼠脑微血管内皮细胞对人参皂苷Rb1的摄取。
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| 关 键 词： | 摄取 根皮素 人参皂苷Rb1 原代大鼠脑微血管内皮细胞 |
| 收稿时间： | 2015-03-09; |
The Effect of Phloretin on rBMECs Uptake of Ginsenoside Rb1 |
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| WANG Yu-zhu,GU Sheng-ying,ZHANG Ji-gang,ZHU Guan-hua,WU Xin,LIU Gao-lin,LI Xiao-yu. The Effect of Phloretin on rBMECs Uptake of Ginsenoside Rb1[J]. Chinese Pharmaceutical Journal, 2015, 50(3): 226-231. DOI: 10.11669/cpj.2015.03.010 |
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| Authors: | WANG Yu-zhu GU Sheng-ying ZHANG Ji-gang ZHU Guan-hua WU Xin LIU Gao-lin LI Xiao-yu |
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| Affiliation: | Department of Clinical Pharmacy, Shanghai First People′s Hospital Affiliated Shanghai Jiaotong University, Shanghai 200080,China |
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| Abstract: | OBJECTIVE To evaluate the effect of phloretin on the uptake of ginsenoside Rb1 by the cell model, primary rat brain microvascular endothelial cells,(rBMEC) in vitro.METHODS Experiments were divided into two groups, ginsenoside Rb1 with and without the addition of phloretin intervention. The cellular uptake of ginsenoside Rb1 was determined by LC-MS/MS. Protein content in cultured cells was measured by the method of BCA. RESULTS The uptake amount of ginsenoside Rb1 in rBMEC with phloretin was significantly lower(P<0.05). CONCLUSION The uptake of ginsenoside Rb1 by rBMEC is significantly inhibited by phloretin. |
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| Keywords: | uptake phloretin ginsenoside Rb1 rBMECs |
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