毛细管电泳同时测定苍耳类药材中7种酚酸类成分的含量

 目的 建立高效毛细管电泳法(HPCE)同时测定苍耳类药材中绿原酸、咖啡酸、阿魏酸、异绿原酸A、异绿原酸C、新绿原酸和1,3-O-二咖啡酰奎宁酸含量的方法。方法 以50 mmol·L^-1硼砂-水(pH 9.54)为缓冲溶液,未涂渍标准熔融石英毛细管(75 μm×64.5 cm, 有效长度56 cm)为分离通道,运行电压为25 kV,检测波长为326 nm,毛细管温度为25 ℃,压力进样为25 kPa×5 s。结果 7种酚酸的浓度与峰面积的线性关系良好(r>0.999 4) ;加样回收率为99.85%~102.70%。用此法测定了苍耳类药材中7种酚酸的含量,得到满意结果。结论 该方法简单、准确,重复性较好,可用于苍耳类药材内在质量的评价和控制。

Simultaneous Determination of Seven Phenolic Acids from Xanthii Herba and Xanthii Fructus by HPCE

OBJECTIVE To establish a high performance capillary electrophoresis (HPCE) method for simultaneousn determination of nchlorogenic acid, caffeic acid, ferulic acid, isochlorogenic acid A,isochlorogenic acid C, neochlorogenic acid, and cynarin in the herbs of Xanthii Herba and Xanthii Fructus. METHODS 50 mmol·L^-1 borax-water was used as buffer solution (pH 9.54). The separation was performed on an uncoated fused silica capillary (64.5 cm×75 μm, 56 cm of effective length) maintained at 25 ℃ at voltage of 25 kV. The detection wavelength was 326 nm, and the sample was injected at 25 kPa×6 s. RESULTS The calibration curves of the seven phenolic acid showed good linearity ( r>0.999 4) in the ranges of the tested concentrations, and the average recoveries of the method were between 99.85%-102.70%. CONCLUSION The method is simple, accurate and reproducible, and can be used for the quality evaluation and control of Xanthii Herba and Xanthii Fructus.