超高效液相色谱双波长法同时测定黄地安消胶囊中5种成分的含量

 目的 采用超高效液相色谱双波长法同时测定黄地安消胶囊中葛根素、大豆苷、大豆苷元、掌叶防己碱、小檗碱的含量。方法 采用Waters ACQUITY UPLC系统,BEH C₁₈(2.1 mm×100 mm,1.7 μm),以乙腈-0.05 mol·L^-1磷酸二氢钾溶液为流动相,梯度洗脱;双波长检测(λ₁=250 nm,λ₂=354 nm);流速:0.20 mL·min^-1;温度:30 ℃。结果 葛根素、大豆苷、大豆苷元、掌叶防已碱、小檗碱的进样量与峰面积分别在0.022～0.176 μg(r=0.999 9)、0.004 3～0.034 4 μg(r=0.999 9)、0.000 61～0.004 9 μg(r=0.999 7)、0.002 3～0.018 4 μg(r=0.999 7)、0.008 6～0.066 8 μg(r=0.999 6)内呈良好的线性关系;平均加样回收率(n=6)分别为97.70%、96.81%、99.36%、99.60%和98.67%,RSD值均小于2.7%。结论 所建立的超高效液相色谱双波长法操作简便,结果准确,重复性好,回收率高,可为黄地安消胶囊的质量评价提供研究基础。

Simultaneous Determination of Five Effective Components in HuangdiAnxiao Capsules by UPLC

OBJECTIVE To establish a UPLC method for simultaneous determination of puerarin, daidzin, daidzine, palmatine, and berberine in HuangdiAnxiao capsules(HDAXC). METHODS The chromatographic analyses were carried out on an ACQUITY UPLC^TM BEH C₁₈ column (2.1 mm×100 mm, 1.7 μm; Waters Corp., USA) maintained at 30 ℃.The mobile phase component A was acetonitrile and B was phosphoric acid with gradient elution at 0.2 mL·min^-1.The five components were detected by dual wavelength, ie λ₁=250 nm,λ₂=354 nm. RESULTS The standard curves of puerarin, daidzin, daidzine, palmatine, and berberine showed good linear relationship in the ranges of 0.022-0.176 μg(r=0.999 9), 0.004 3-0.034 4 μg(r=0.999 9), 0.000 61-0.004 9 μg(r=0.999 7), 0.002 3-0.018 4 μg(r=0.999 7), and 0.008 6-0.066 8 μg(r=0.999 6),respectively. The average recoveries were 97.70%, 96.81%, 99.36%, 99.60% and 98.67% with RSDs of less than 2.7%(n=6). CONCLUSION This method is simple, rapid, accurate, and reproducible, and it can lay basis for the quality evaluation of HDAXC.