The effect of psoralen plus ultraviolet A in vitro in HUT-78 enhances by 5-aminolevulinic acid |
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Authors: | Akita Yoichi Watanabe Daisuke Yanagishita Takeshi Kuhara Tomoe Kawamura Chiharu Masuda Yuko Kawada Morihiro Nakaseko Hirono Tamada Yasuhiko Matsumoto Yoshinari |
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Affiliation: | Department of Dermatology, Aichi Medical University School of Medicine, Nagakute, Japan. 106039@gk.amu.aichi-med-u.ac.jp |
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Abstract: | BACKGROUND: Sezary syndrome and mycosis fungoides are forms of cutaneous T-cell lymphoma, and in the early stage of these diseases psoralen plus ultraviolet A (PUVA) is one of the treatments of choice. Photodynamic therapy using 5-aminolevulinic acid (ALA-PDT) is an effective, non-invasive, and safe treatment for most superficial skin cancers. In order to obtain greater efficacy of PUVA, we investigated the synergistic anti-tumor effects of ALA-PDT and PUVA using 8-methoxypsoralen (8-MOP) and a UVA lamp. METHODS: The in vitro effects of PUVA and ALA-PDT and their combination in HUT-78 cell line from human SS were determined by MTT assay. RESULTS: In our results, cell proliferation compared with controls was inhibited to 53.2% with UVA alone, 52.3% with 1 microM 8-MOP, 43.8% with 100 microM ALA, and 19.2% with combined 8-MOP and ALA. CONCLUSION: Combined use of ALA and PUVA using 8-MOP and UVA lamps, which are widespread in Japan, had a strong anti-tumor effect in vitro. Combined treatment with ALA-PDT and PUVA using a UVA lamp appears to have a strong treatment effect. |
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Keywords: | 5-aminolevulinic acid cutaneous T cell lymphoma photodynamic therapy protoporphyrin IX psoralen plus ultraviolet A |
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