| 反相高效液相色谱-荧光法测定血清中异丙酚浓度 |
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| 引用本文： | 杜文力,王明霞,邢玉英,宋晓靖,宗矩. 反相高效液相色谱-荧光法测定血清中异丙酚浓度[J]. 中国药房, 2002, 13(2): 96-97 |
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| 作者姓名： | 杜文力 王明霞 邢玉英 宋晓靖 宗矩 |
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| 作者单位： | 1. 河北医科大学第四医院,石家庄市,050011
2. 河北医科大学药学院99届毕业生,石家庄市,050017 |
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| 摘 要： | 目的 :建立反相高效液相色谱 -荧光检测法测定血清中异丙酚浓度的方法。方法 :使用ODSC18 柱 ,以甲醇 -水 (75∶25)为流动相 ,激发波长270nm ,发射波长295nm。结果 :本法测定的线性范围为0 0375～8μg/ml ,r=0 9996 ,日内和日间RSD<5 % ,平均回收率为83 98 %。结论 :本法简单、快速 ,适用于手术过程中异丙酚的药物浓度监测
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| 关 键 词： | 异丙酚 血药浓度 反相高效液相色谱法 |
| 文章编号： | 1001-0408(2002)02-0096-02 |
| 修稿时间： | 2001-03-02 |
Determination of Propofol Concentration in Human Serum With RP- HPLC |
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| DU Wenli,WANG Mingxia,XING Yuying. Determination of Propofol Concentration in Human Serum With RP- HPLC[J]. China Pharmacy, 2002, 13(2): 96-97 |
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| Authors: | DU Wenli WANG Mingxia XING Yuying |
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| Abstract: | OBJECTIVE:To develop a RP-HPLC method for determining serum concentration of propofol METHODS:Using ODS C18 column as fixed phase,a mixture of methanol and water(75∶25) as mobile phase,excitation wavelength 270nm,emission wavelength 295nm RESULTS:The linear range was 0 0 375～8 0μg/ml,r=0 9 996 The within-day and between-day RSDs were less than 5%,the average recovery was 83 98% CONCLUSION:This is a good method to monitor propofol serum concentration |
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| Keywords: | propofol serum concentration of drug RP-HPLC |
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