肺癌易感性的分子流行病学研究

目的 分析GSTP1基因型与肺癌易感性的关系,探讨肺癌的敏感基因型,为肺癌易感者的筛检和肺癌病人的早期诊断提供依据,作好肺癌的二级预防措施.方法 应用病例对照研究方法与基因体外扩增限制性片段长度多态性分析技术相结合的分子流行病学研究,检测GSTP1基因型的分布频率,分析GSTP1基因多态性与肺癌易感性的关系,评价GSTP1突变基因型对肺癌发病的影响.结果 分析326例GSTP1基因多态性,其中病例组158例,对照组168例.总体上病例组与对照组的AA、AG和GG基因型的分布频率的差异无显着的统计学意义;50岁以上不吸烟的男性、含有GSTP1突变基因型(AG或GG)者在病例组和对照组中的分布频率的差异有非常显着的统计学意义;GSTP1突变基因型与吸烟对肺癌的发生不存在交互作用.结论 50岁以上的男性、含有GSTP1突变基因型的人患肺癌的危险性比不合GSTP1突变基因型者大83倍.

Genetic Polymorphism of Glutathione S - transferase P1 Gene and Susceptibility to Lung Cancer

Objective To analyse the relationship between the GSTP1 genotypes and susceptibility to lung cancer,and explore the sensitive genotype of lung neoplasm through evaluating on the intension of the association between GSTP1 gene and lung cancer and to provide basis for screen and diagnosis of lung cancer.Methods The molecular epidemiological studies was adopted.A case-control study was carried out,with 158 cases and 168 controls;Polymerase Chain Reaction-Restriction Fragment Length Polymorphism(PCR-RFLP)was taken to analyse the GSTP1 genotypes and identify the gene frequencies.Results The difference of GSTP1 genotypes distribution were not statistically significant between cases and controls.After excluding the confounding of smoking in the elder age subgroup(age 50 years or elder),the frequencies of GSTP1 variant genotypes(AG or GG)in the cases were evidently higher than that in the controls.Conclusion In the elder age subgroup(age 50 years or elder),the risk to lung cancer was 8.3 times higher among the males carrying G alleles(AG and GG)and than those carrying A alleles(AA)with the exception of smoking.