Magnetosonoporation: Instant magnetic labeling of stem cells |
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| Authors: | Bensheng Qiu Daohai Xie Piotr Walczak Xubin Li Jesus Ruiz‐Cabello Satoshi Minoshima Jeff WM Bulte Xiaoming Yang |
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| Institution: | 1. Image‐Guided Bio‐Molecular Interventions Section, Department of Radiology, University of Washington School of Medicine, Seattle, Washington, USA;2. Department of Radiology, Suzhou University School of Medicine, Jiangsu, China;3. Division of MR Research, Russell H. Morgan Department of Radiology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA;4. Department of Chemical and Biomolecular Engineering, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA;5. Department of Radiology, Peking University People's Hospital, Beijing, China;6. Instituto de Estudios Biofuncionales and CIBER Enfermedades Respiratorias, Universidad Complutense de Madrid, Spain;7. Department of Biomedical Engineering, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA;8. Cellular Imaging Section, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA |
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| Abstract: | The purpose of this study was to develop an instant MR cell labeling technique, called magnetosonoporation. First, a magnetosonoporation apparatus was successfully established for MR labeling of stem cells. Then, the safety of this new cell labeling approach was confirmed by evaluation of cell viability, proliferation, and differentiation of magnetosonoporation‐labeled and unlabeled C17.2 neural stem cells. Subsequently, the feasibility of using in vivo MRI to detect magnetosonoporation/Feridex‐labeled stem cells was validated in living animals and confirmed by histologic correlation. The magnetosonoporation technique is expected to be convenient, efficient, and safe for future clinical application of MRI‐guided cell therapies. Magn Reson Med 63:1437–1441, 2010. © 2010 Wiley‐Liss, Inc. |
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| Keywords: | MRI stem cells magnetosonoporation superpara‐magnetic iron oxide particles MR cell labeling |
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