| 人胚胎胰腺组织干细胞的分离鉴定和纯化 |
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| 引用本文： | 葛焕琦,蔡寒青,门秀丽,许世清,潘焕峰,娄晋宁. 人胚胎胰腺组织干细胞的分离鉴定和纯化[J]. 中华糖尿病杂志, 2007, 15(7): 437-439 |
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| 作者姓名： | 葛焕琦 蔡寒青 门秀丽 许世清 潘焕峰 娄晋宁 |
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| 作者单位： | [1]吉林大学第二医院内分泌科,长春130041 [2]卫生部中日友好医院临床医学研究所,长春130041 [3]吉林市第二中心医院内分泌科,长春130041 |
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| 基金项目： | 国家高技术研究发展计划(863计划);吉林省科技厅科研项目 |
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| 摘 要： | 分离人胚胎胰腺组织中的胰岛细胞于体外培养,应用免疫细胞化学及RT-PCR的方法对其中的胰腺干细胞进行鉴定,并应用免疫磁珠的方法纯化其中的干细胞。结论是人胚胎胰腺组织中存在一定比例的干细胞,可以作为体外向胰岛素分泌细胞诱导分化的细胞来源。
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| 关 键 词： | 胰腺干细胞 糖尿病 诱导分化 胰岛移植 |
| 收稿时间： | 2007-04-27 |
| 修稿时间： | 2007-04-27 |
Identification and purification of human fetal pancreatic stem cells |
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| GE Huan-qi , CAI han-qing,MEN Xiu-li,et al.. Identification and purification of human fetal pancreatic stem cells[J]. CHINESE JOURNAL OF DIABETES MELLITUS, 2007, 15(7): 437-439 |
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| Authors: | GE Huan-qi CAI han-qing MEN Xiu-li et al. |
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| Affiliation: | Department of Endocrinology, the Second Hospital of Jilin University, Changchun 130041 , China |
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| Abstract: | Pancreatic islet cells were isolated from human fetal pancreatic tissue and identified through immunocytochemical methods and RT-PCR. Then pancreatic stem cells were purified using nestin antibody-conjugated magnetic beads. In conclusion, there are a certain proportion of pancreatic stem cells existing in human fetal pancreatic tissue, and they could be the resources of cell induction and differentiation into insulin producing cells. |
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| Keywords: | Pancreatic stem cell Diabetes mellitus Differentiation Islet transplantation |
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