辛伐他汀/聚己内酯静电纺丝膜的制备及其生物相容性评价

目的：制备辛伐他汀（simvastatin）/聚己内酯（Polycaprolactone,PCL）静电纺丝膜,评价其生物相容性.方法：制备PCL静电纺丝膜和simvastatin/PCL静电纺丝膜,通过扫描电镜观察两种膜片的形貌特征.选择人牙周膜成纤维细胞（periodontal ligament fibroblasts,PDLFs）与膜片浸提液共培养,检测细胞增殖情况,评价材料毒性;PDLFs接种于膜片共培养7d,扫描电镜观察细胞生长情况,以直接接触法检测膜片的细胞毒性.将PCL和simvastatin/PCL静电纺丝膜植入大鼠皮下,术后14d取出标本,HE和Masson染色法进行组织学观察.结果：成功制备出纤维直径为纳米级别的simvastatin/PCL静电纺丝膜.细胞增殖试验和直接接触试验结果显示,PCL和simvastatin/PCL静电纺丝膜的浸提液细胞毒性试验均合格,细胞在材料上伸展充分,连接成片.PCL和simvastatin/PCL静电纺丝膜植入皮下2周后均有包膜形成;与PCL静电纺丝膜相比,simvastatin/PCL静电纺丝膜与周围组织界限较明显,反应层炎症细胞较少.结论：simvastatin/PCL静电纺丝膜的生物相容性良好,细胞毒性和组织相容性均符合生物支架材料的要求.

Biocompatibility of electrospun simvastatin / PCL membrane scaffolds

Objective： To prepare electrospun simvastatin/PCL membrane scaffolds and to evaluate the biocompatibility in vitro and in vivo. Method： Electrospun PCL membrane and simvastatin/PCL membrane scaffolds were prepared. The morphology and characteristics of membrane surface were observed with scanning electron microscopy. Human PDLFs were incubated with the scaffold diffusion solution and cell proliferation was measured. PDLFs were seeded on the scaffolds and cell adhesion to membrane was observed with scanning electron microscopy at day7. PCL and simvastatin / PCL membrane scaffolds were respectively implanted subcutaneously into rats. Specimens were harvested at dayl4 for gross observation and histological examination through HE and Masson staining. Result： PCL nanofibers containing simvastatin were successfully electrospun. Test of cell adhesion and proliferation displayed that both PCL and simvastatin / PCL membrane scaffolds sup- ported adhesion and proliferation of PDLFs,which were capable of spanning the nearby struts. Fibrous tissue encapsulating both subcutaneously implanted membrane scaffolds after dayl4 was noted, and increasing transparency with reducing inflam- matory cells were indicated in simvastatin / PCL membrane scaffolds. Conclusion： Electrospun simvastatin / PCL membrane demonstrated acceptable biocompatibility in vitro and in vivo, and it could be a safe and prospective scaffold in periodontal tissue engineering.