口服壳聚糖胰岛素纳米粒的制备及其降血糖作用研究

目的研究口服壳聚糖胰岛素纳米粒的制备方法及其降血糖作用.方法以壳聚糖为包被材料,用离子交联法制备壳聚糖胰岛素纳米粒,用透射电镜和纳米粒度分析仪测定纳米粒形态和粒径,以Wistar大鼠为动物模型,研究胰岛素纳米粒口服后对健康和糖尿病大鼠的降血糖作用.结果制备得到的纳米粒多呈球形,粒径为220.6±15.9nm,胰岛素包封率为75.4%±3.2%,载药量为19.5%±2.6%.降血糖实验表明健康大鼠在灌胃(25U/kg)后6～12h内,血糖浓度显著降低;糖尿病大鼠在灌胃(25U/kg)后6h血糖开始下降,这种降血糖作用可维持9h以上,其中血糖维持在正常水平的时间可达7h.结论壳聚糖胰岛素纳米粒对健康和糖尿病大鼠都具有一定的降血糖作用.

Preparation and blood sugar lowering effect of oral chitosan-insulin nanoparticles on diabetic rats

Objective To study the method of preparation and blood sugar lowering effect of oral chitosan-insulin nanoparticles (INS-NPs) in streptozotocin-induced diabetic Wistar rats. Methods The INS-NPs were prepared by an ionic gelation method. The changes in the morphology and size of the INS-NPs were observed with transmission electron microscope and Zetasizer 3000HS, respectively. The blood sugar lowering effect of the INS-NPs was evaluated by monitoring the blood glucose levels in healthy and streptozotocin-induced diabetic rats. Results INS-NPs were spherical in shape with a mean size of 220.6±15.9nm. Entrapment efficiency of INS-NPs was 75.4%±3.2% and the loading efficiency of INS-NPs was 19.5%±2.6%. In vivo blood sugar lowering study showed that the levels of blood glucose of healthy Wistar rats were significantly reduced from 6h to 12h after oral administration of INS-NPs(25U/kg). The blood glucose level of diabetic rats were significantly reduced at 6h after oral administration of INS-NPs (25U/kg), and this effect was maintained for more than 9h, and the levels of blood glucose were kept in normal range for 7h. Conclusion The INS-NPs prepared by ionic gelation method has the blood glucose lowering effect in streptozotocin-induced diabetic Wistar rats.